S-腺苷蛋氨酸在肝硬變大鼠行部分肝葉切除后對肝功能的保護作用觀察_《中國普外基礎與臨床雜志》

作者：

張捷 ¹ , 廖傳文 ² , 朱洪 ¹ , 田大廣 ¹ , 郭永章 ¹

1.昆明醫學院第二附屬醫院肝膽外科（昆明650101）;;
2.江西省第一人民醫院普通外科（南昌330000）;

關鍵詞：

S-腺苷蛋氨酸肝硬變肝葉切除術肝再生腫瘤壞死因子-α

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目的探討S腺苷蛋氨酸在肝硬變大鼠行部分肝葉切除后對肝細胞的再生及肝功能的影響。方法用40%四氯化碳誘導Wistar大鼠肝硬變模型，而后將成模大鼠隨機分為肝硬變組（n=20）、治療1組〔S腺苷蛋氨酸10 mg/（kg·d），n=16〕及治療2組〔S腺苷蛋氨酸20 mg/（kg·d），n=16〕，另設正常對照組（n=16）。4組大鼠均行30%左右肝葉切除。自手術當天起，治療1、2組分別肌注S腺苷蛋氨酸10 mg/（kg·d）和20 mg/（kg·d），對照組肌注生理鹽水5 ml/d，共15 d。4組大鼠分別于術后15 d和30 d處死一半，取血檢測Alb、ALT、TB、TBA及TNFα，同時在光鏡及電鏡下觀察肝組織的病理改變及超微結構變化。結果肝硬變組術后15 d和 30 d的TB、TBA、ALT及TNFα水平明顯高于正常對照組（P＜0.01）, Alb水平明顯低于正常對照組（P＜0.01）；治療1、2組術后 15 d的上述4項指標高于正常對照組(P＜0.05)，但明顯低于肝硬變組（P＜0.01），Alb水平低于正常對照組（P＜0.05），卻明顯高于肝硬變組（P＜0.01）；治療1、2組術后30 d上述各指標均接近正常對照組水平。TNFα和TB、TBA、ALT呈顯著正相關（r值分別為 0.99、0.97、0.93， P＜0.01），與Alb呈顯著負相關（r值為-0.88，P＜0.01）。治療組光鏡觀察見肝小葉結構基本完整，有較多的雙核肝細胞以及少量淋巴細胞浸潤，電鏡觀察見粗面內質網及糖原增生，線粒體豐富。結論S腺苷蛋氨酸能促進肝葉切除術后肝細胞的再生，可改善肝功能。

引用本文： 張捷,廖傳文,朱洪,田大廣,郭永章. S-腺苷蛋氨酸在肝硬變大鼠行部分肝葉切除后對肝功能的保護作用觀察. 中國普外基礎與臨床雜志, 2003, 10(4): 332-335. doi: 復制

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11.	繆明永，蔡在龍，劉鵬飛,等.大鼠在肝再生過程中線粒體氧化磷酸化的調控 [Ｊ]. 中國病理生理雜志，1999; 15(5)∶398.

1. 2Higgins GM. Experimental pathology of liver restoration of the liver of the white rat following partial surgical removal [Ｊ]. Aech Pathol, 1991; 12(2)∶186.
2. 3Almasio P, Pagliaro L. Ademetionine: the state of the art and future prospects [Ｊ]. Ann Ital Med Int， 1993; 8(1 Suppl)∶52S.
3. 4VarelaMoreiras G, AlonsoAperte E, Rubio M,et al. Carbon tetrachlorideinduced hepatic injury is associated with global DNA hypomethylation and homocysteinemia: effect of Sadenosylmethionine treatment [Ｊ]. Hepatology, 1995; 22(4 Pt 1)∶1310.
4. 5Holecek M, Skopec F, Sprongl L. Influence of buthionine sulfoximine, Sadenosylmethionine and glutathione on liver regeneration following partial hepatectomy [Ｊ]. Arzneimittelforschung， 2000; 50(12)∶1093.
5. 6Mato JM, Corrales FJ, Lu SC,et al. SAdenosylmethionine: a control switch that regulates liver function [Ｊ].FASEB J, 2002; 16(1)∶15.
6. 7Feo F,Garcea R,Daino L,et al. Early stimulation of polyamine biosynthesis during promotion by phenobartial of diethylnitrosanineinduced rat liver carcinogenesis. The effect of variations of the SadenosylLmethionine cellular pool [Ｊ].Carcinogenesis,1985; 6(12)∶1713.
7. 8Lieber CS. Role of oxidative stress and antioxidant therapy in alcoholic and nonalcoholicliver diseases [Ｊ]. Adv Pharmacol， 1997; 38(8)∶601.
8. 9Drahota Z, Rauchova H, Sedlak V, et al. The effect of triiodothyronine on changes of membrane fluidity in regenerating rat liver [Ｊ]. Physiol Res， 1999; 48(2)∶167.
9. 11Watson WH,Zhao Y,Chawla RK.Sadenosylmethionine attenuates the lipopolysaccharideinduced expression of the gene for tumour necrosis alpha [Ｊ].Biochem J, 1999; 342(Pt 1)∶21.
10. 孫自勤，王要軍，權啟鎮，等. 肝纖維化大鼠血清有機磷酸酯酶的變化 [Ｊ].新消化病雜志， 1994; 2（4）∶206.
11. 繆明永，蔡在龍，劉鵬飛,等.大鼠在肝再生過程中線粒體氧化磷酸化的調控 [Ｊ]. 中國病理生理雜志，1999; 15(5)∶398.

《中國普外基礎與臨床雜志》

S-腺苷蛋氨酸在肝硬變大鼠行部分肝葉切除后對肝功能的保護作用觀察

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《中國普外基礎與臨床雜志》

S-腺苷蛋氨酸在肝硬變大鼠行部分肝葉切除后對肝功能的保護作用觀察

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

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Content

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