肝移植大鼠肝竇內皮細胞的損傷及PGE1對其的保護作用_《中國普外基礎與臨床雜志》

作者：

田根東 ¹ , 何延政 ¹ , 張艷敏 ² , 何春水 ¹

1.瀘州醫學院附屬醫院血管外科（瀘州646000）;;
2.瀘州醫學院組織胚胎學教研室;

關鍵詞：

肝移植肝竇內皮細胞前列腺素E1

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目的探討肝移植大鼠術后早期肝竇內皮細胞功能的改變及前列腺素E1（PGE1）對其的保護作用。方法用“兩袖套法”建立大鼠原位肝移植模型，其中A組為正常大鼠空白對照組，B組為正常大鼠供體肝移植手術對照組，C組為休克性大鼠供體肝移植實驗對照組，D組為PGE1保護的休克性大鼠供體肝移植實驗組，每組各8只。肝移植各組（無特別說明均指受體）于術后6 h取血，檢測其血清中的肝臟酶學（ALT、LDH）、丙二醛（MDA）和一氧化氮（NO），以及血漿內皮素（ET）水平，并取肝組織作常規病理學檢查，比較各組有無差異。結果各移植組供肝冷保存時間及無肝期均接近，分別為（2±0.5） h和（15±3） min；　B、D組術后6 h全部存活，存活率100%； C組術后6 h存活5只，存活率62.5%。與C組比較，D組ALT、LDH、MDA、ET明顯降低（P＜0.05），NO明顯升高（P＜0.05 ）。C組肝臟病理學檢查發現有明顯的組織學損害，而B、D組肝臟組織結構基本完好。結論肝移植可造成肝竇內皮細胞明顯損害，血清NO和血漿ET水平可較好地反映肝竇內皮細胞的功能狀態。PGE1通過穩定血流動力學和穩定肝竇內皮細胞質膜，可減輕移植肝肝竇內皮細胞的損害。

引用本文： 田根東,何延政,張艷敏,何春水. 肝移植大鼠肝竇內皮細胞的損傷及PGE1對其的保護作用. 中國普外基礎與臨床雜志, 2003, 10(4): 328-331. doi: 復制

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8.	9季德剛，劉丙義，鄭澤霖，等．前列腺素E1對大鼠肝臟再灌注損傷的保護作用 [Ｊ].中國普外基礎與臨床雜志， 2003; 10(1)∶ 36.
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10.	11Kishimoto S, Sakon M, Umeshita K, et al. The inhibitory effect of prostaglandin E1 on oxidative stressinduced hepatocyte injury evaluated by calpainμ activation [Ｊ]. Transplantation, 2000; 69(11)∶2314.
11.	12Middleton A, Middleton B. Elevation of cyclic AMP by iloprost and prostaglandin E1 increases cholesterol efflux and the binding capacity for highdensity lipoproteins in human fibroblasts [Ｊ].Biochim Biophys Acta, 1998; 1391(2)∶117.
12.	嚴以群，楊甲梅，王軒，等．大鼠減體積肝移植模型的初步研究 [Ｊ].中華器官移植雜志， 1996； 17(2)∶55.

1. 1Kamada N, Calne RY. Orthotopic liver transplantation in the rat. Technique using cuff for portal vein anastomosis and biliary drainage [Ｊ].Transplantation, 1979; 28(1)∶47.
2. 3 Menger MD, Richter S, Yamauchi J, et al. Role of microcirculation in hepatic ischemia/reperfusion injury [Ｊ]. Hepatogastroenterology, 1999; 46(Suppl 2)∶1452.
3. 4Imamura H, Brault A, Huet PM. Effects of extended cold preservation and transplantation on the rat liver microcirculation [Ｊ]. Hepatology, 1997; 25(3)∶664.
4. 5Mitsuoka H，Suzuki S，Sakaguchi T，et al．Contribution of endothelin1 to microcirculatory impairment in total hepatic ischemia and reperfusion injury [Ｊ]. Transplantation, 1999; 67(4)∶514.
5. 6SerracinoInglott F, Habib NA, Mathie RT. Hepatic ischemiareperfusion injury [Ｊ]. Am J Surg, 2001; 181(2)∶ 160.
6. 7 Itasaka H, Suehiro T, Wakiyama S, et al. The mechanism of hepatic graft protection against reperfusion injury by prostaglandin E1 [Ｊ]. Surg Today, 1999; 29(6)∶526.
7. 8Oishi M, Tanaka N, Orita K. Beneficial effects of prostaglandin E1 on hemodynamic changes during liver transplantation in pigs [Ｊ]. Transpl Int, 1996; 9(Suppl 1)∶S100.
8. 9季德剛，劉丙義，鄭澤霖，等．前列腺素E1對大鼠肝臟再灌注損傷的保護作用 [Ｊ].中國普外基礎與臨床雜志， 2003; 10(1)∶ 36.
9. 10Xu HS, Rosenlof LK, Pruett TL, et al. Prostaglandin E1 increases survival with extended anhepatic phase during liver transplantation [Ｊ]. Ann Surg, 1994; 220(1)∶53.
10. 11Kishimoto S, Sakon M, Umeshita K, et al. The inhibitory effect of prostaglandin E1 on oxidative stressinduced hepatocyte injury evaluated by calpainμ activation [Ｊ]. Transplantation, 2000; 69(11)∶2314.
11. 12Middleton A, Middleton B. Elevation of cyclic AMP by iloprost and prostaglandin E1 increases cholesterol efflux and the binding capacity for highdensity lipoproteins in human fibroblasts [Ｊ].Biochim Biophys Acta, 1998; 1391(2)∶117.
12. 嚴以群，楊甲梅，王軒，等．大鼠減體積肝移植模型的初步研究 [Ｊ].中華器官移植雜志， 1996； 17(2)∶55.

《中國普外基礎與臨床雜志》

肝移植大鼠肝竇內皮細胞的損傷及PGE1對其的保護作用

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《中國普外基礎與臨床雜志》

肝移植大鼠肝竇內皮細胞的損傷及PGE1對其的保護作用

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

Format

Content

摘要全文圖表視頻參考文獻施引文獻補充材料