高效液相色譜電化學法檢測尿液8羥基脫氧鳥苷水平_《中國普外基礎與臨床雜志》

作者：

彭濤 ¹ , 嚴律南 ¹ , 劉志明 ² , 葛西宏 ³ , 彭民浩 ² , 黎樂群 ²

1.四川大學華西醫院普外科（成都610041）;;
2.廣西醫科大學附一院普外科（南寧530021）;;
3.日本產業醫科大學生態科學研究所腫瘤部（Kitakyushu 807-8555，Japan）;;
4.廣西醫科大學附一院檢驗科;;
5.廣西扶綏縣腫瘤防治研究所（扶綏532100）;

關鍵詞：

高效液相色譜電化學8羥基脫氧鳥苷肝癌

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的探討一套高效液相色譜電化學系統檢測尿8羥基脫氧鳥苷(8OHdG)水平的方法學。方法采用ESA 5600 Coularray系統檢測廣西扶綏縣肝癌戶人群21例及南寧市對照人群63例尿8OHdG水平。結果8OHdG峰與雜質峰分離良好； 8OHdG濃度在0.01～15 ng/ml間呈良好線性；保留時間、標樣和內對照的重復性試驗的批內差異(CV)均小于8%；標樣(F=0.002, P=1.000)和內對照(F=2.965, P=0.082)的重復性試驗的批間差異經方差分析均無顯著性意義。采用上述方法測得84例總的尿8OHdG水平為（5.26±0.41）ng/mg肌酐，高于同類研究的檢測值〔（4.62±0.091）ng/mg肌酐, n=32〕，但兩地人群間尿8OHdG水平差異無顯著性意義。結論這是對肝癌高發區高危人群氧化性DNA損傷的首次報告。通過謹慎設計試驗和優化試驗參數，該系統對8OHdG峰的分離優于以往報告，適用于尿8OHdG的高通量定量分析，可應用于人群氧化性ＤＮＡ損傷的研究。

引用本文： 彭濤,嚴律南,劉志明,葛西宏,彭民浩,黎樂群. 高效液相色譜電化學法檢測尿液8羥基脫氧鳥苷水平. 中國普外基礎與臨床雜志, 2003, 10(3): 281-283. doi: 復制

1.	Halliwell B. Free radical, antioxidants, and human disease: curiosity, cause, or consequence? [J]. Lancet, 1994； 344(13)∶721.
2.	Grollman AP, Moriya M. Mutagenesis by 8oxoguanine: an enemy within [J]. Trends Genet, 1993； 9(7)∶246.
3.	Kasai H. Analysis of a form of oxidative DNA damage, 8hydroxy2’deoxyguanosine, as a marker of cellular oxidative stress during carcinogenesis [J]. Mutat Res, 1997； 387(3)∶147.
4.	Kasai H, IwamotoTanaka N, Miyamoto T, et al. Life style and urinary 8hydroxydeoxyguanosine, a marker of oxidative dna damage: effects of exercise, working conditions, meat intake, body mass index, and smoking [J]. Jpn J Cancer Res, 2001； 92(1)∶9.
5.	Bogdanov MB, Beal MF, McCabe DR, et al. A carbon columnbased liquid chromatography electrochemical approach to routine 8hydroxy2’deoxyguanosine measurements in urine and other biologic matrices: a oneyear evaluation of methods [J]. Free Radic Biol Med, 1999； 27(5-6)∶647.
6.	Tagesson C, Chabiuk D, Axelson O,et al. Increased urinary excretion of the oxidative DNA adduct, 8hydroxydeoxyguanosine, as a possible early indicator of occupational cancer hazards in the asbestos, rubber, and azodye industies [J]. Pol J Occup Med Environ Health, 1993； 6(4)∶357.
7.	Loft S, Vistisen K, Ewertz M, et al. Oxidative DNA damage estimated by 8hydroxydeoxyguanosine excretion in humans: influence of smoking, gender and body mass index [J]. Carcinogenesis, 1992； 13(12)∶2241.
8.	Acworth IN, McCabe DR, Maher TJ. The analysis of free radical, their reaction products, and antioxidants. In: Baskin SI, Salem H. Eds. Oxidants, antioxidants, and free radicals [M]. Washington: Taylor & Francis， 1997∶23-77.

1. Halliwell B. Free radical, antioxidants, and human disease: curiosity, cause, or consequence? [J]. Lancet, 1994； 344(13)∶721.
2. Grollman AP, Moriya M. Mutagenesis by 8oxoguanine: an enemy within [J]. Trends Genet, 1993； 9(7)∶246.
3. Kasai H. Analysis of a form of oxidative DNA damage, 8hydroxy2’deoxyguanosine, as a marker of cellular oxidative stress during carcinogenesis [J]. Mutat Res, 1997； 387(3)∶147.
4. Kasai H, IwamotoTanaka N, Miyamoto T, et al. Life style and urinary 8hydroxydeoxyguanosine, a marker of oxidative dna damage: effects of exercise, working conditions, meat intake, body mass index, and smoking [J]. Jpn J Cancer Res, 2001； 92(1)∶9.
5. Bogdanov MB, Beal MF, McCabe DR, et al. A carbon columnbased liquid chromatography electrochemical approach to routine 8hydroxy2’deoxyguanosine measurements in urine and other biologic matrices: a oneyear evaluation of methods [J]. Free Radic Biol Med, 1999； 27(5-6)∶647.
6. Tagesson C, Chabiuk D, Axelson O,et al. Increased urinary excretion of the oxidative DNA adduct, 8hydroxydeoxyguanosine, as a possible early indicator of occupational cancer hazards in the asbestos, rubber, and azodye industies [J]. Pol J Occup Med Environ Health, 1993； 6(4)∶357.
7. Loft S, Vistisen K, Ewertz M, et al. Oxidative DNA damage estimated by 8hydroxydeoxyguanosine excretion in humans: influence of smoking, gender and body mass index [J]. Carcinogenesis, 1992； 13(12)∶2241.
8. Acworth IN, McCabe DR, Maher TJ. The analysis of free radical, their reaction products, and antioxidants. In: Baskin SI, Salem H. Eds. Oxidants, antioxidants, and free radicals [M]. Washington: Taylor & Francis， 1997∶23-77.

《中國普外基礎與臨床雜志》

高效液相色譜電化學法檢測尿液8羥基脫氧鳥苷水平

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《中國普外基礎與臨床雜志》

高效液相色譜電化學法檢測尿液8羥基脫氧鳥苷水平

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Content

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