攜反義二型基質金屬蛋白酶基因的重組腺病毒的構建_《中國普外基礎與臨床雜志》

作者：

張明滿 ¹ , 李德華 ² , 茍興華 ² , 嚴律南 ¹ , 韓蕾 ² , 趙蘭英 ² , 胡海洋 ²

1.四川大學華西醫院普外科（成都610041）;;
2.成都地奧制約集團有限公司新藥部（成都610041）;;
通迅作者：嚴律南;

關鍵詞：

肝細胞肝癌二型基質金屬蛋白酶重組腺病毒反義RNA技術基因治療

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目的構建攜帶反義二型基質金屬蛋白酶（MMP2）基因的重組腺病毒。
方法從新鮮肝癌組織中提取總RNA,用RT-PCR法合成MMP2 cDNA序列中5′端轉錄起始位點附近長約500 bp的基因片段，將此片段反義克隆到腺病毒載體(AdEasy)系統的多克隆位點，經轉染293細胞生成攜帶反義MMP2基因的重組腺病毒AdMMP2AS。
結果成功構建并包裝攜帶反義MMP2基因片段的重組腺病毒Ad-MMP2AS，病毒滴度達1×108/ml。
結論構建的重組腺病毒Ad-MMP2AS可望能有效地將反義MMP2基因片段導入人肝癌細胞株，為進一步研究肝癌浸潤和轉移機理以及探討抑制肝癌浸潤和轉移的方法提供實驗基礎。

引用本文： 張明滿,李德華,茍興華,嚴律南,韓蕾,趙蘭英,胡海洋. 攜反義二型基質金屬蛋白酶基因的重組腺病毒的構建. 中國普外基礎與臨床雜志, 2004, 11(5): 428-432. doi: 復制

1.	Bu W, Huang X, Tang Z. The role of MMP2 in the invasion and metastasis of hepatocellular carcinoma (HCC) [Ｊ]. Zhonghua Yi Xue Za Zhi, 1997; 77(9)∶661.
2.	Niu Q, Tang Z, Ma Z, et al. Relationship between serum matrix metalloproteinase2 and metastasis and recurrence following radical hepatic resection in hepatocellular carcinoma [Ｊ]. Zhonghua Gan Zang Bing Za Zhi, 2001; 9（Suppl）∶58.
3.	王愛民，江龍安，梁保忠，等. 原發性肝癌組織中明膠酶A基因表達增強 [Ｊ]. 中華普通外科雜志， 2001； 15（9）∶598.
4.	He TC, Zhou S, da Costa LT, et al. A simplified system for generating recombinant adenoviruses [Ｊ]. Proc Natl Acad Sci USA， 1998; 95(5)∶ 2509.
5.	Curran S, Murray GI. Matrix metalloproteinases in tumour invasion and metastasis [Ｊ]. J Pathol， 1999; 189(3)∶ 300.
6.	Liotta LA, Tryggvason K, Garbisa S, et al. Metastatic potential correlates with enzymatic degradation of basement membrane collagen [Ｊ]. Nature， 1980; 284(5751)∶ 67.
7.	Deryugina EI, Luo GX, Reisfeld RA, et al. Tumor cell invasion through matrigel is regulated by activated matrix metalloproteinase2 [Ｊ]. Anticancer Res， 1997; 17(5A)∶ 3201.
8.	Sang QX. Complex role of matrix metalloproteinases in angiogenesis [Ｊ]. Cell Res， 1998; 8(3)∶ 171.
9.	McKenna GJ, Chen Y, Smith RM, et al. A role for matrix metalloproteinases and tumor host interaction in hepatocellular carcinomas [Ｊ]. Am J Surg， 2002; 183(5)∶ 588.
10.	Carter G, Lemoine NR. Antisense technology for cancer therapy: does it make sense? [Ｊ]. Br J Cancer, 1993; 67(5)∶ 869.
11.	Lakka SS, Rajan M, Gondi C, et al. Adenovirusmediated expression of antisense MMP9 in glioma cells inhibits tumor growth and invasion [Ｊ]. Oncogene, 2002; 21(52)∶ 8011.

1. Bu W, Huang X, Tang Z. The role of MMP2 in the invasion and metastasis of hepatocellular carcinoma (HCC) [Ｊ]. Zhonghua Yi Xue Za Zhi, 1997; 77(9)∶661.
2. Niu Q, Tang Z, Ma Z, et al. Relationship between serum matrix metalloproteinase2 and metastasis and recurrence following radical hepatic resection in hepatocellular carcinoma [Ｊ]. Zhonghua Gan Zang Bing Za Zhi, 2001; 9（Suppl）∶58.
3. 王愛民，江龍安，梁保忠，等. 原發性肝癌組織中明膠酶A基因表達增強 [Ｊ]. 中華普通外科雜志， 2001； 15（9）∶598.
4. He TC, Zhou S, da Costa LT, et al. A simplified system for generating recombinant adenoviruses [Ｊ]. Proc Natl Acad Sci USA， 1998; 95(5)∶ 2509.
5. Curran S, Murray GI. Matrix metalloproteinases in tumour invasion and metastasis [Ｊ]. J Pathol， 1999; 189(3)∶ 300.
6. Liotta LA, Tryggvason K, Garbisa S, et al. Metastatic potential correlates with enzymatic degradation of basement membrane collagen [Ｊ]. Nature， 1980; 284(5751)∶ 67.
7. Deryugina EI, Luo GX, Reisfeld RA, et al. Tumor cell invasion through matrigel is regulated by activated matrix metalloproteinase2 [Ｊ]. Anticancer Res， 1997; 17(5A)∶ 3201.
8. Sang QX. Complex role of matrix metalloproteinases in angiogenesis [Ｊ]. Cell Res， 1998; 8(3)∶ 171.
9. McKenna GJ, Chen Y, Smith RM, et al. A role for matrix metalloproteinases and tumor host interaction in hepatocellular carcinomas [Ｊ]. Am J Surg， 2002; 183(5)∶ 588.
10. Carter G, Lemoine NR. Antisense technology for cancer therapy: does it make sense? [Ｊ]. Br J Cancer, 1993; 67(5)∶ 869.
11. Lakka SS, Rajan M, Gondi C, et al. Adenovirusmediated expression of antisense MMP9 in glioma cells inhibits tumor growth and invasion [Ｊ]. Oncogene, 2002; 21(52)∶ 8011.

《中國普外基礎與臨床雜志》

攜反義二型基質金屬蛋白酶基因的重組腺病毒的構建

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《中國普外基礎與臨床雜志》

攜反義二型基質金屬蛋白酶基因的重組腺病毒的構建

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

Format

Content

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