奧曲肽對人胃癌細胞的凋亡誘導作用_《中國普外基礎與臨床雜志》

作者：

1.大連友誼醫院外二科（大連116001）;;
2.安徽醫科大學第一附屬醫院普外科（合肥230022）;

關鍵詞：

奧曲肽細胞凋亡胃癌SGC7901細胞

視頻：

導出 下載 收藏 掃碼 引用

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

目的探討生長抑素對人胃癌細胞的抑制作用及其作用機理。方法以生長抑素類似物奧曲肽作用于人胃癌細胞株SGC7901細胞，以人成纖維細胞株HF作為正常細胞對照，以5FU作為陽性藥物對照，通過MTT實驗、熒光顯微鏡觀察及流式細胞術分析，得出結論。結果一定濃度范圍內的奧曲肽對SGC7901細胞產生抑制作用，其抑制作用具有量效關系和飽和性。奧曲肽對SGC7901細胞的抑制程度接近于5FU對他的抑制，而對HF細胞無影響。奧曲肽可誘導SGC7901細胞凋亡。結論奧曲肽在體外對SGC7901細胞具有抑制作用，誘導腫瘤細胞凋亡可能是其作用機理之一。

引用本文： 呂文才,耿小平,孟翔,凌譚,文翔. 奧曲肽對人胃癌細胞的凋亡誘導作用. 中國普外基礎與臨床雜志, 2004, 11(3): 238-241. doi: 復制

1.	Goldberg RM, Moertel CG, Wieand HS, et al. A phase Ⅲ evaluation of a somatostatin analogue (octreotide) in the treatment of patients with asymptomatic advanced colon carcinoma. North Central Cancer Treatment Group and the Mayo Clinic [Ｊ]. Cancer, 1995; 76(6)∶ 961.
2.	Tomassetti P, Migliori M, Gullo L. Slowrelease lanreotide treatment in endocrine gastrointestinal tumors [Ｊ]. Am J Gastroenterol， 1998; 93(9)∶1468.
3.	Eriksson B, Renstrup J, Imam H, et al. Highdose treatment with lanreotide of patients with advanced neuroendocrine gastrointestinal tumors: clinical and biological effects [Ｊ]. Ann Oncol， 1997; 8(10)∶1041.
4.	Madeira I, Ruszniewski P. Digestive system carcinoid tumors: treatment [Ｊ]. Rev Med Interne， 1999; 20(5)∶421.
5.	鄭永唐，賁昆龍. 測定細胞存活和增殖的MTT方法的建立 [Ｊ]. 免疫學雜志， 1992； 8（4）∶266.
6.	Arnold R, Trautmann ME, Creutzfeldt W, et al. Somatostatin analogue octreotide and inhibition of tumour growth in metastatic endocrine gastroenteropancreatic tumours [Ｊ]. Gut， 1996; 38(3)∶430.
7.	Degen L, Beglinger C. The role of octreotide in the treatment of gastroenteropancreatic endocrine tumors [Ｊ]. Digestion， 1999; 60（Suppl 2）∶9.
8.	鄂征主編. 組織培養和分子細胞學技術 [M]. 第1版. 北京：北京出版社， 1995∶203～205.
9.	Di Leo A, Bajetta E, Ferrari L, et al. A dosefinding study of lanreotide (a somatostatin analog) in patients with colorectal carcinoma [Ｊ]. Cancer, 1996; 78(1)∶35.
10.	Janson ET, Stridsberg M, Gobl A, et al. Determination of somatostatin receptor subtype 2 in carcinoid tumors by immunohistochemical investigation with somatostatin receptor subtype 2 antibodies [Ｊ]. Cancer Res, 1998; 58(11)∶2375.
11.	Qin Y, Ertl T, Groot K, et al. Somatostatin analog RC160 inhibits growth of CFPAC1 human pancreatic cancer cells in vitro and intracellular production of cyclic adenosine monophosphate [Ｊ]. Int J Cancer, 1995; 60(5)∶694.
12.	Reisine T. Somatostatin receptors [Ｊ]. Am J Physiol， 1995; 269(6 Pt 1)∶G813.
13.	吳蘇冬，劉長利，王慧川，等. 參臼膠囊誘導肝癌SMMC7721細胞凋亡 [Ｊ]. 世界華人消化雜志， 2003； 11（7）∶908.

1. Goldberg RM, Moertel CG, Wieand HS, et al. A phase Ⅲ evaluation of a somatostatin analogue (octreotide) in the treatment of patients with asymptomatic advanced colon carcinoma. North Central Cancer Treatment Group and the Mayo Clinic [Ｊ]. Cancer, 1995; 76(6)∶ 961.
2. Tomassetti P, Migliori M, Gullo L. Slowrelease lanreotide treatment in endocrine gastrointestinal tumors [Ｊ]. Am J Gastroenterol， 1998; 93(9)∶1468.
3. Eriksson B, Renstrup J, Imam H, et al. Highdose treatment with lanreotide of patients with advanced neuroendocrine gastrointestinal tumors: clinical and biological effects [Ｊ]. Ann Oncol， 1997; 8(10)∶1041.
4. Madeira I, Ruszniewski P. Digestive system carcinoid tumors: treatment [Ｊ]. Rev Med Interne， 1999; 20(5)∶421.
5. 鄭永唐，賁昆龍. 測定細胞存活和增殖的MTT方法的建立 [Ｊ]. 免疫學雜志， 1992； 8（4）∶266.
6. Arnold R, Trautmann ME, Creutzfeldt W, et al. Somatostatin analogue octreotide and inhibition of tumour growth in metastatic endocrine gastroenteropancreatic tumours [Ｊ]. Gut， 1996; 38(3)∶430.
7. Degen L, Beglinger C. The role of octreotide in the treatment of gastroenteropancreatic endocrine tumors [Ｊ]. Digestion， 1999; 60（Suppl 2）∶9.
8. 鄂征主編. 組織培養和分子細胞學技術 [M]. 第1版. 北京：北京出版社， 1995∶203～205.
9. Di Leo A, Bajetta E, Ferrari L, et al. A dosefinding study of lanreotide (a somatostatin analog) in patients with colorectal carcinoma [Ｊ]. Cancer, 1996; 78(1)∶35.
10. Janson ET, Stridsberg M, Gobl A, et al. Determination of somatostatin receptor subtype 2 in carcinoid tumors by immunohistochemical investigation with somatostatin receptor subtype 2 antibodies [Ｊ]. Cancer Res, 1998; 58(11)∶2375.
11. Qin Y, Ertl T, Groot K, et al. Somatostatin analog RC160 inhibits growth of CFPAC1 human pancreatic cancer cells in vitro and intracellular production of cyclic adenosine monophosphate [Ｊ]. Int J Cancer, 1995; 60(5)∶694.
12. Reisine T. Somatostatin receptors [Ｊ]. Am J Physiol， 1995; 269(6 Pt 1)∶G813.
13. 吳蘇冬，劉長利，王慧川，等. 參臼膠囊誘導肝癌SMMC7721細胞凋亡 [Ｊ]. 世界華人消化雜志， 2003； 11（7）∶908.

《中國普外基礎與臨床雜志》

奧曲肽對人胃癌細胞的凋亡誘導作用

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

Format

Content

《中國普外基礎與臨床雜志》

奧曲肽對人胃癌細胞的凋亡誘導作用

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

Format

Content

摘要全文圖表視頻參考文獻施引文獻補充材料