急性胰腺炎大鼠肝臟NFκB對TNFα表達的調控及其在肝損傷中的作用_《中國普外基礎與臨床雜志》

作者：

石力 ¹ , 田伏洲 ¹ , 李旭 ¹ , 黃大熔 ¹ , 趙碧 ² , 顧大勇 ²

1.成都軍區總醫院全軍普外中心（成都610083）;;
2. 成都軍區總醫院臨床實驗科;

關鍵詞：

急性胰腺炎核因子κB腫瘤壞死因子α肝臟損傷

視頻：

導出 下載 收藏 掃碼 引用

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

目的觀察急性胰腺炎發生時肝組織中核因子κB（NFκB）對TNFα mRNA表達的調節及其在肝損傷中的作用。方法Wistar大鼠72只，隨機均分為急性胰腺炎組（AP組）、急性胰腺炎吡咯基二硫氨基甲酸酯（PDTC）處理組（APP組）以及對照組（SO組）。分別在術后3 h、6 h、12 h及24 h檢測肝組織NFκB活性、TNFα mRNA的表達以及血漿ALT水平。結果AP組及APP組的NFκB活性、TNFα mRNA表達及血漿ALT水平分別在術后3～6 h及3～24 h顯著高于SO組。在應用了NFκB抑制劑的APP組，NFκB活性、TNFα mRNA表達以及血漿ALT水平均顯著低于AP組。結論急性胰腺炎發生時，肝臟中活化的NFκB促進了TNFα mRNA的表達，并參與了肝損傷的發生。

引用本文： 石力,田伏洲,李旭,黃大熔,趙碧,顧大勇. 急性胰腺炎大鼠肝臟NFκB對TNFα表達的調控及其在肝損傷中的作用. 中國普外基礎與臨床雜志, 2004, 11(3): 228-230. doi: 復制

1.	Miyahara S, Isaji S. Liver injury in acute pancreatitis and mitigation by continuous arterial infusion of an antibiotic via the superior mesenteric artery [Ｊ]. Pancreas, 2001; 23(2)∶204.
2.	石力，黃大熔，田伏洲，等. 急性胰腺炎大鼠肝臟核因子κB活化與肝損傷的關系 [Ｊ]. 世界華人消化雜志， 2002； 10（5）∶554.
3.	Jaffray C, Yang J, Norman J. Elastase mimics pancreatitisinduced hepatic injury via inflammatory mediators [Ｊ]. J Surg Res, 2000; 90(2)∶95.
4.	Deryckere F, Gannon F. A onehour minipreparation technique for extraction of DNAbinding proteins from animal tissues [Ｊ]. Biotechniques, 1994; 16(3)∶405.
5.	Chomczynski P, Sacchi N. Singlestep method of RNA isolation by acid guanidinium thiocyanatephenolchloroform extraction [Ｊ]. Anal Biochem, 1987; 162(1)∶156.
6.	Pitchumoni CS, Agarwal N, Jain NK. Systemic complications of acute pancreatitis [Ｊ]. Am J Gastroenterol, 1988; 83(6)∶597.
7.	Valen G, Yan ZQ, Hansson GK. Nuclear factor kappaB and the heart [Ｊ]. J Am Coll Cardiol, 2001; 38(2)∶307.
8.	Wright G, Singh IS, Hasday JD, et al. Endotoxin stressresponse in cardiomyocytes: NFkappa B activation and tumor necrosis factoralpha expression [Ｊ]. Am J Physiol Heart Circ Physiol, 2002; 282(3)∶H872.
9.	Moine P, McIntyre R, Schwartz MD, et al. NFkappa B regulatory mechanisms in alveolar macrophages from patients with acute respiratory distress syndrome [Ｊ]. Shock, 2000; 13(2)∶85.
10.	Udalova IA, Knight JC, Vidal V, et al. Complex NFkappa B interactions at the distal tumor necrosis factor promoter region in human monocytes [Ｊ]. J Biol Chem, 1998; 273(33)∶21178.
11.	Essani NA, Fisher MA, Jaeschke H. Inhibition of NFkappa B activation by dimethyl sulfoxide correlates with suppression of TNFalpha formation, reduced ICAM1 gene transcription, and protection against endotoxininduced liver injury [Ｊ]. Shock, 1997; 7(2)∶90.

1. Miyahara S, Isaji S. Liver injury in acute pancreatitis and mitigation by continuous arterial infusion of an antibiotic via the superior mesenteric artery [Ｊ]. Pancreas, 2001; 23(2)∶204.
2. 石力，黃大熔，田伏洲，等. 急性胰腺炎大鼠肝臟核因子κB活化與肝損傷的關系 [Ｊ]. 世界華人消化雜志， 2002； 10（5）∶554.
3. Jaffray C, Yang J, Norman J. Elastase mimics pancreatitisinduced hepatic injury via inflammatory mediators [Ｊ]. J Surg Res, 2000; 90(2)∶95.
4. Deryckere F, Gannon F. A onehour minipreparation technique for extraction of DNAbinding proteins from animal tissues [Ｊ]. Biotechniques, 1994; 16(3)∶405.
5. Chomczynski P, Sacchi N. Singlestep method of RNA isolation by acid guanidinium thiocyanatephenolchloroform extraction [Ｊ]. Anal Biochem, 1987; 162(1)∶156.
6. Pitchumoni CS, Agarwal N, Jain NK. Systemic complications of acute pancreatitis [Ｊ]. Am J Gastroenterol, 1988; 83(6)∶597.
7. Valen G, Yan ZQ, Hansson GK. Nuclear factor kappaB and the heart [Ｊ]. J Am Coll Cardiol, 2001; 38(2)∶307.
8. Wright G, Singh IS, Hasday JD, et al. Endotoxin stressresponse in cardiomyocytes: NFkappa B activation and tumor necrosis factoralpha expression [Ｊ]. Am J Physiol Heart Circ Physiol, 2002; 282(3)∶H872.
9. Moine P, McIntyre R, Schwartz MD, et al. NFkappa B regulatory mechanisms in alveolar macrophages from patients with acute respiratory distress syndrome [Ｊ]. Shock, 2000; 13(2)∶85.
10. Udalova IA, Knight JC, Vidal V, et al. Complex NFkappa B interactions at the distal tumor necrosis factor promoter region in human monocytes [Ｊ]. J Biol Chem, 1998; 273(33)∶21178.
11. Essani NA, Fisher MA, Jaeschke H. Inhibition of NFkappa B activation by dimethyl sulfoxide correlates with suppression of TNFalpha formation, reduced ICAM1 gene transcription, and protection against endotoxininduced liver injury [Ｊ]. Shock, 1997; 7(2)∶90.

《中國普外基礎與臨床雜志》

急性胰腺炎大鼠肝臟NFκB對TNFα表達的調控及其在肝損傷中的作用

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

Format

Content

《中國普外基礎與臨床雜志》

急性胰腺炎大鼠肝臟NFκB對TNFα表達的調控及其在肝損傷中的作用

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

Format

Content

摘要全文圖表視頻參考文獻施引文獻補充材料