褪黑素對大鼠梗阻性黃疸腎損害的保護作用_《中國普外基礎與臨床雜志》

作者：

張小明 ¹ , 寇治民 ¹ , 康二虎 ² , 張有成 ¹

1.蘭州大學第二醫院普外科（蘭州730030）;;
2.蘭州大學第二醫院急診外科;

關鍵詞：

梗阻性黃疸腎功能障礙一氧化氮誘生性一氧化氮合酶褪黑素

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【摘要】目的探討一氧化氮（NO）在大鼠梗阻性黃疸腎功能損害發生中的作用，以及褪黑素（melatonin,Mel)的保護作用。
方法64只雄性SD大鼠隨機均分為4組：正常對照組（CN組）、假手術組（SO組）、膽總管結扎組（BLD組）和褪黑素治療組（BDL+Mel組）。應用膽總管結扎法建立梗阻性黃疸模型，分別于手術后第4天和第8天兩個時間點檢測4組中血漿NO、TB、DB、ALT、AST、BUN、Cr水平以及腎組織中NO水平和iNOS活性；光鏡下觀察腎組織病理形態改變。
結果BDL組大鼠血漿NO、TB、DB、ALT、AST、BUN、Cr水平和腎組織NO水平及iNOS活性明顯升高（P＜0.01），褪黑素治療可使血漿NO、ALT、AST、BUN及Cr水平和腎組織中NO水平及iNOS活性顯著降低，與BDL組比較差異有顯著性意義（P＜0.01）。
結論梗阻性黃疸時，腎組織iNOS活性增強，NO大量產生參與了腎功能損害的發生、發展；褪黑素對大鼠梗阻性黃疸腎損害的保護作用至少部分是通過干擾NO通路實現的。

引用本文： 張小明,寇治民,康二虎,張有成. 褪黑素對大鼠梗阻性黃疸腎損害的保護作用. 中國普外基礎與臨床雜志, 2005, 12(1): 55-58. doi: 復制

1.	GonzalezCorrea JA, De La Cruz JP, MartinAurioles E, et al. Effects of SadenosylLmethionine on hepatic and renal oxidative stress in an experimental model of acute biliary obstruction in rats [Ｊ]. Hepatology, 1997; 26(1)∶121.
2.	劉祥德，李衛，何振平，等. NO與梗阻性黃疸時腎損害的關系 [Ｊ]. 第二軍醫大學學報， 2001； 23（7）∶795.
3.	Gilad E, Wong HR, Zingarelli B, et al. Melatonin inhibits expression of the inducible isoform of nitric oxide synthase in murine macrophages: role of inhibition of NFkappaB activation [Ｊ]. FASEB J, 1998; 12(9)∶685.
4.	Crespo E, Macias M, Pozo D, et al. Melatonin inhibits expression of the inducible NO synthase II in liver and lung and prevents endotoxemia in lipopolysaccharideinduced multiple organ dysfunction syndrome in rats [Ｊ]. FASEB J, 1999; 13(12)∶1537.
5.	Dugo L, Serraino I, Fulia F, et al. Effect of melatonin on cellular energy depletion mediated by peroxynitrite and poly (ADPribose) synthetase activation in an acute model of inflammation [Ｊ]. J Pineal Res, 2001; 31(1)∶76.
6.	Nath KA, Norby SM. Reactive oxygen species and acute renal failure [Ｊ].Am J Med， 2000; 109(8)∶ 665.
7.	Kannan K, Jain SK. Oxidative stress and apoptosis [Ｊ].Pathophysiology， 2000; 7(3)∶153.
8.	Jaeschke H. Reactive oxygen and mechanisms of inflammatory liver injury [Ｊ].J Gastroenterol Hepatol， 2000; 15(7)∶718.
9.	Ohta Y, Kongo M, Kishikawa T. Melatonin exerts a therapeutic effect on cholestatic liver injury in rats with bile duct ligation [Ｊ]. J Pineal Res, 2003; 34(2)∶119.
10.	Cruz A, Padillo FJ, Tunez I, et al. Melatonin protects against renal oxidative stress after obstructive jaundice in rats [Ｊ]. Eur J Pharmacol, 2001; 425(2)∶135.
11.	Chen CY, Shiesh SC, Tsao HC, et al. Protective effect of melatonin on renal injury of rats induced by bile duct ligation [Ｊ].Dig Dis Sci, 2001; 46(4)∶927.

1. GonzalezCorrea JA, De La Cruz JP, MartinAurioles E, et al. Effects of SadenosylLmethionine on hepatic and renal oxidative stress in an experimental model of acute biliary obstruction in rats [Ｊ]. Hepatology, 1997; 26(1)∶121.
2. 劉祥德，李衛，何振平，等. NO與梗阻性黃疸時腎損害的關系 [Ｊ]. 第二軍醫大學學報， 2001； 23（7）∶795.
3. Gilad E, Wong HR, Zingarelli B, et al. Melatonin inhibits expression of the inducible isoform of nitric oxide synthase in murine macrophages: role of inhibition of NFkappaB activation [Ｊ]. FASEB J, 1998; 12(9)∶685.
4. Crespo E, Macias M, Pozo D, et al. Melatonin inhibits expression of the inducible NO synthase II in liver and lung and prevents endotoxemia in lipopolysaccharideinduced multiple organ dysfunction syndrome in rats [Ｊ]. FASEB J, 1999; 13(12)∶1537.
5. Dugo L, Serraino I, Fulia F, et al. Effect of melatonin on cellular energy depletion mediated by peroxynitrite and poly (ADPribose) synthetase activation in an acute model of inflammation [Ｊ]. J Pineal Res, 2001; 31(1)∶76.
6. Nath KA, Norby SM. Reactive oxygen species and acute renal failure [Ｊ].Am J Med， 2000; 109(8)∶ 665.
7. Kannan K, Jain SK. Oxidative stress and apoptosis [Ｊ].Pathophysiology， 2000; 7(3)∶153.
8. Jaeschke H. Reactive oxygen and mechanisms of inflammatory liver injury [Ｊ].J Gastroenterol Hepatol， 2000; 15(7)∶718.
9. Ohta Y, Kongo M, Kishikawa T. Melatonin exerts a therapeutic effect on cholestatic liver injury in rats with bile duct ligation [Ｊ]. J Pineal Res, 2003; 34(2)∶119.
10. Cruz A, Padillo FJ, Tunez I, et al. Melatonin protects against renal oxidative stress after obstructive jaundice in rats [Ｊ]. Eur J Pharmacol, 2001; 425(2)∶135.
11. Chen CY, Shiesh SC, Tsao HC, et al. Protective effect of melatonin on renal injury of rats induced by bile duct ligation [Ｊ].Dig Dis Sci, 2001; 46(4)∶927.

《中國普外基礎與臨床雜志》

褪黑素對大鼠梗阻性黃疸腎損害的保護作用

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《中國普外基礎與臨床雜志》

褪黑素對大鼠梗阻性黃疸腎損害的保護作用

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

Format

Content

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