尼美舒利對結腸腺癌細胞MMP-2表達的影響_《中國普外基礎與臨床雜志》

作者：

劉偉 ¹ , 安杰 ² , 張超 ¹

1. 第三軍醫大學西南醫院普外科（重慶400038）;;
2.北京軍區白求恩國際和平醫院病理科（河北石家莊050082）;

關鍵詞：

結腸腫瘤環氧合酶抑制劑基質金屬蛋白酶

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【摘要】目的探討選擇性環氧合酶2（COX2）抑制劑——尼美舒利對體外培養的結腸癌細胞的生長及對基質金屬蛋白酶2（MMP2）表達的影響。方法實驗分為尼美舒利組、二甲亞砜（DMSO）對照組及不接種細胞的空白對照組，采用MTT法檢測不同濃度的尼美舒利對人結腸癌細胞株HT29(COX2高表達)及HCT116(COX2低表達)增殖的抑制效應；采用改良明膠酶譜分析法檢測MMP2的表達。結果尼美舒利對人結腸癌細胞株HT29和HCT116生長的抑制作用呈時間、劑量依賴性，且對HT29細胞的抑制作用強于HCT116細胞；尼美舒利抑制了HT29細胞的MMP2表達，而對HCT116細胞MMP2的表達的抑制作用不明顯。結論尼美舒利可明顯抑制COX2高表達的結腸癌細胞株HT29的增殖和生長，提示尼美舒利可能通過抑制COX2活性而降低細胞MMP2的表達。

引用本文： 劉偉,安杰,張超. 尼美舒利對結腸腺癌細胞MMP-2表達的影響. 中國普外基礎與臨床雜志, 2005, 12(6): 585-588. doi: 復制

1 Fujita T, Matsui M, Takaku K, et al. Size  and invasiondependent increase in cyclooxygenase 2 levels in human colorectal carcinomas [Ｊ]. Cancer Res， 1998; 58(21)48232 Mook OR, Frederiks WM, Van Noorden CJ. The role of gelatinases in colorectal cancer progression and metastasis [Ｊ]. Biochim Biophys Acta, 2004; 1705(2)693 Reddy BS, Rao CV. Colon cancer: a role for cyclooxygenase2specific nonsteroidal antiinflammatory drugs [Ｊ]. Drugs Aging, 2000; 16(5)3294 Tsujii M, DuBois RN. Alterations in cellular adhesion and apoptosis in epithelial cells overexpressing prostaglandin endoperoxide synthase 2 [Ｊ]. Cell, 1995; 83(3)4935 Piazza GA, Rahm AK, Finn TS, et al. Apoptosis primarily accounts for the growthinhibitory properties of sulindac metabolites and involves a mechanism that is independent of cyclooxygenase inhibition, cell cycle arrest, and p53 induction [Ｊ]. Cancer Res, 1997; 57(12)24526 Shiff SJ, Qiao L, Tsai LL, et al. Sulindac sulfide, an aspirinlike compound, inhibits proliferation, causes cell cycle quiescence, and induces apoptosis in HT29 colon adenocarcinoma cells [Ｊ]. J Clin Invest, 1995; 96(1)4917 Kleiner DE, StetlerStevenson WG. Matrix metalloproteinases and metastasis [Ｊ]. Cancer Chemother Pharmacol, 1999; 43(Suppl)S428 Wu J, Akaike T, Hayashida K, et al. Enhanced vascular permeability in solid tumor involving peroxynitrite and matrix metalloproteinases [Ｊ]. Jpn J Cancer Res, 2001; 92(4)4399 Oba K, Konno H, Tanaka T, et al. Prevention of liver metastasis of human colon cancer by selective matrix metalloproteinase inhibitor MMI166 [Ｊ]. Cancer Lett, 2002; 175(1)4510 Papadopoulou S, Scorilas A, Arnogianaki N, et al. Expression of gelatinaseA (MMP2) in human colon cancer and normal colon mucosa [Ｊ]. Tumour Biol, 2001; 22(6)38311 Ko K, Yazumi S, Yoshikawa K, et al. Activation of fibroblastderived matrix metalloproteinase2 by coloncancer cells in noncontact Cocultures [Ｊ]. Int J Cancer, 2000; 87(2)16512 Li G, Yang T, Yan J. Cyclooxygenase-2 increased the angiogenic and metastatic potential of tumor cells [Ｊ]. Biochem Biophys Res Commun， 2002; 299(5)886.

1. 1 Fujita T, Matsui M, Takaku K, et al. Size  and invasiondependent increase in cyclooxygenase 2 levels in human colorectal carcinomas [Ｊ]. Cancer Res， 1998; 58(21)48232 Mook OR, Frederiks WM, Van Noorden CJ. The role of gelatinases in colorectal cancer progression and metastasis [Ｊ]. Biochim Biophys Acta, 2004; 1705(2)693 Reddy BS, Rao CV. Colon cancer: a role for cyclooxygenase2specific nonsteroidal antiinflammatory drugs [Ｊ]. Drugs Aging, 2000; 16(5)3294 Tsujii M, DuBois RN. Alterations in cellular adhesion and apoptosis in epithelial cells overexpressing prostaglandin endoperoxide synthase 2 [Ｊ]. Cell, 1995; 83(3)4935 Piazza GA, Rahm AK, Finn TS, et al. Apoptosis primarily accounts for the growthinhibitory properties of sulindac metabolites and involves a mechanism that is independent of cyclooxygenase inhibition, cell cycle arrest, and p53 induction [Ｊ]. Cancer Res, 1997; 57(12)24526 Shiff SJ, Qiao L, Tsai LL, et al. Sulindac sulfide, an aspirinlike compound, inhibits proliferation, causes cell cycle quiescence, and induces apoptosis in HT29 colon adenocarcinoma cells [Ｊ]. J Clin Invest, 1995; 96(1)4917 Kleiner DE, StetlerStevenson WG. Matrix metalloproteinases and metastasis [Ｊ]. Cancer Chemother Pharmacol, 1999; 43(Suppl)S428 Wu J, Akaike T, Hayashida K, et al. Enhanced vascular permeability in solid tumor involving peroxynitrite and matrix metalloproteinases [Ｊ]. Jpn J Cancer Res, 2001; 92(4)4399 Oba K, Konno H, Tanaka T, et al. Prevention of liver metastasis of human colon cancer by selective matrix metalloproteinase inhibitor MMI166 [Ｊ]. Cancer Lett, 2002; 175(1)4510 Papadopoulou S, Scorilas A, Arnogianaki N, et al. Expression of gelatinaseA (MMP2) in human colon cancer and normal colon mucosa [Ｊ]. Tumour Biol, 2001; 22(6)38311 Ko K, Yazumi S, Yoshikawa K, et al. Activation of fibroblastderived matrix metalloproteinase2 by coloncancer cells in noncontact Cocultures [Ｊ]. Int J Cancer, 2000; 87(2)16512 Li G, Yang T, Yan J. Cyclooxygenase-2 increased the angiogenic and metastatic potential of tumor cells [Ｊ]. Biochem Biophys Res Commun， 2002; 299(5)886.

《中國普外基礎與臨床雜志》

尼美舒利對結腸腺癌細胞MMP-2表達的影響

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《中國普外基礎與臨床雜志》

尼美舒利對結腸腺癌細胞MMP-2表達的影響

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

Format

Content

摘要全文圖表視頻參考文獻施引文獻補充材料