通過抑制ERK1/2通路誘導肝癌細胞SMMC-7721凋亡的研究_《中國普外基礎與臨床雜志》

作者：

盛薇 ¹ , 王康 ¹ , 平高峰 ¹ , 李徐奇 ¹ , 魏光兵 ¹ , 黃辰 ²

1.西安交通大學醫學院第一附屬醫院普外科（西安710061）;;
2.西安交通大學環境與腫瘤相關基因教育部重點實驗室生物醫學基礎研究中心（西安710061）;

關鍵詞：

細胞外信號調節激酶肝癌細胞株增殖凋亡

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目的　應用細胞外信號調節激酶(ERK)1/2抑制劑U0126研究ERK1/2在肝癌細胞增殖、凋亡中的作用。
方法　以肝癌SMMC-7721細胞株為材料，分為空白對照組及不同濃度的U0126處理組。以MTT檢測細胞增殖情況，流式細胞儀分析細胞周期及細胞凋亡情況。
結果　不同濃度的U0126處理后均可明顯抑制肝癌SMMC-7721細胞的增殖（P＜0.05，P＜0.01）,使處于G0/G1期的細胞明顯增多（P＜0.05）且呈劑量依賴性，并誘發細胞凋亡發生（P＜0.05）。
結論　阻斷ERK1/2通路有可能成為肝癌治療的重要手段。

引用本文： 盛薇,王康,平高峰,李徐奇,魏光兵,黃辰. 通過抑制ERK1/2通路誘導肝癌細胞SMMC-7721凋亡的研究. 中國普外基礎與臨床雜志, 2007, 14(6): 675-678. doi: 復制

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1. 　Bos JL. All in the family? New insights and questions regarding interconnectivity of Ras, Rap1 and Ral [J]. EMBO J， 1998; 17(23)∶6776.
2. 　Steinmetz R, Wagoner HA, Zeng P, et al. Mechanisms regulating the constitutive activation of the ERK signaling pathway in ovarian cancer and the effect of RNAi for ERK1/2 on cancer cell proliferation [J]. Mol Endocrino, 2004; 15(3)∶1004.
3. 　Wu TT, Wang JS, Jiaan BP, et al. Role of p21(WAF1) and p27(KIP1) in predicting biochemical recurrence for organ-confined prostate adenocarcinoma [J]. J Chin Med Assoc， 2007; 70(1)∶11.
4. 　Handra-Luca A, Bilal H, Bertrand JC, et al. Extra-cellular signal-regulated ERK-1/ERK-2 pathway activation in human salivary gland mucoepidermoid carcinoma: association to aggressive tumor behavior and tumor cell proliferation [J]. Am J Pathol， 2003; 163(3)∶957.
5. 　Hoshino R, Chatani Y, Yamori T, et al. Constitutive activation of the 41-/43-kDa mitogen-activated protein kinase signaling pathway in human tumors [J]. Oncogene， 1999; 18(3)∶813.
6. 　Mishima K, Inoue K, Hayashi Y. Overexpression of extracellular-signal regulated kinases on oral squamous cell carcinoma [J]. Oral Oncol， 2002; 38(5)∶468.
7. 　鄭銘，張幼怡，韓啟德. β腎上腺素受體的絲裂原活化蛋白激酶信號途徑 [J]. 生理科學進展, 2003; 33(2)∶111.
8. 　Kolch W. Meaningful relationships: the regulation of the Ras/Raf/MEK/ERK pathway by protein interactions [J]. Biochem J， 2000; 351（Pt 2）∶289.
9. 　Gysin S, Lee SH, Dean NM, et al. Pharmacologic inhibition of RAF→MEK→ERK signaling elicits pancreatic cancer cell cycle arrest through induced expression of p27Kip1 [J]. Cancer Res， 2005; 65(11)∶4870.
10. 　Ito Y, Sasaki Y, Horimoto M, et al. Activation of mitogen-activated protein kinases/extracellular signal-regulated kinases in human hepatocellular carcinoma [J]. Hepatology， 1998; 27(4)∶951.
11. 　Adeyinka A, Nui Y, Cherlet T, et al. Activated mitogen-activated protein kinase expression during human breast tumorigenesis and breast cancer progression [J]. Clin Cancer Res， 2002; 8(6)∶1747.
12. 　Albanell J, Codony-Servat J, Rojo F, et al. Activated extracellular signal-regulated kinases: association with epidermal growth factor receptor/transforming growth factor alpha expression in head and neck squamous carcinoma and inhibition by anti-epidermal growth factor receptor treatments [J]. Cancer Res， 2001; 61(17)∶6500.
13. 　Uzgare AR, Kaplan PJ, Greenberg NM. Differential expression and/or activation of P38MAPK, erk1/2, and jnk during the initiation and progression of prostate cancer [J]. Prostate， 2003; 55(2)∶128.
14. 　Rincon-Arano H, Rosales R, Mora N, et al. R-Ras promotes tumor growth of cervical epithelial cells [J]. Cancer， 2003; 97(3)∶575.
15. 　Gioeli D, Mandell JW, Petroni GR, et al. Activation of mitogen-activated protein kinase associated with prostate cancer progression [J]. Cancer Res， 1999; 59(2)∶279.
16. 　Anders M, Christian C, McMahon M, et al. Inhibition of the Raf/MEK/ERK pathway up-regulates expression of the coxsackievirus and adenovirus receptor in cancer cells [J]. Cancer Res， 2003; 63(9)∶2088.
17. 　Wen J, Zhang Y, Chen X, et al. Enhancement of diallyl disulfide-induced apoptosis by inhibitors of MAPKs in human HepG2 hepatoma cells [J]. Biochem Pharmacol， 2004; 68(2)∶323.
18. 　黃辰，劉利英，宋土生，等. siRNA介導的MAPK p42沉默誘導HeLa細胞凋亡 [J]. 南方醫科大學學報， 2006; 26(1)∶11.
19. 　Tamemoto H, Kadowaki T, Tobe K, et al. Biphasic activation of two mitogen-activated protein kinases during the cell cycle in mammalian cells [J]. J Biol Chem， 1992; 267(28)∶20293.
20. 　黃辰，劉利英，宋土生，等. 使用技術抑制細胞P42MAPK表達 [J]. 中華病理學雜志, 2006； 35(5)∶292.

《中國普外基礎與臨床雜志》

通過抑制ERK1/2通路誘導肝癌細胞SMMC-7721凋亡的研究

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《中國普外基礎與臨床雜志》

通過抑制ERK1/2通路誘導肝癌細胞SMMC-7721凋亡的研究

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

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Content

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