金雀異黃素與5-氟尿嘧啶對SGC-7901 細胞的抑制作用_《中國普外基礎與臨床雜志》

作者：

王正文 ¹ , 朱明才 ² , 湯為學 ³ , 龔軍 ⁴ , 范維珂 ³ , 魏錦來 ² , 崔海寧 ¹

1. 海南醫學院附屬醫院(海口570102) ;;
2. 重慶醫科大學附屬第一醫院普外科(重慶400016) ;;
3. 重慶醫科大學基礎醫學院病理生理教研室(重慶400016) ;;
4. 四川省廣安市人民醫院普外科(廣安638000);

關鍵詞：

胃癌,SGC27901 細胞,金雀異黃素,5-氟尿嘧啶,抑制作用

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目的　研究金雀異黃素( genistein , Gen) 與52氟尿嘧啶(52fluorouracil ,52FU) 對人胃癌細胞SGC-7901 的抑制作用。方法　采用MTT 法檢測Gen 和5-FU 對SGC-7901 細胞的抑制作用,流式細胞術檢測細胞周期分布,并在透射電鏡下觀察細胞超微結構改變。結果　Gen 和5-FU 單用或聯合應用對胃癌SGC27901 細胞的生長均有顯著的增殖抑制作用,且呈劑量和時間依賴性。兩藥聯合應用時,當藥物效應在0. 2～0. 8 時,具有協同或相加作用(CI ≤1) ; 18. 8μmol/ L 的Gen 作用后,62. 97 %的SGC-7901 細胞阻滯在G2 / M 期; 8. 84μmol/ L 的52FU作用后,63. 76 %的SGC-7901 細胞阻滯在S 期; 3. 06 μmol/ L 的Gen 與7. 96 μmol/ L 的5-FU 聯合應用后,67. 46 %的SGC27901 細胞阻滯在G0 / G1 期。Gen 和52FU 均可誘導SGC-7901 細胞凋亡。結論　Gen 與5-FU 通過阻滯細胞周期和誘導細胞凋亡而抑制胃癌細胞的生長,對胃癌細胞的增殖抑制具有協同作用。

引用本文： 王正文,朱明才,湯為學,龔軍,范維珂,魏錦來,崔海寧. 金雀異黃素與5-氟尿嘧啶對SGC-7901 細胞的抑制作用. 中國普外基礎與臨床雜志, 2008, 15(1): 17-22. doi: 復制

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2.	龔軍,王正文,朱明才,等.金雀異黃素和5-FU對人結腸癌細胞株(SW480)的相互作用的體外觀察[J].中國現代醫學雜志,2006; 16(6)∶873.
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5.	Shon YH,Park SD,Nam KS.Effective chemopreventive activity of genistein against human breast cancer cells[J].J Biochem Mol Biol,2006; 39(4)∶448.
6.	Yu Z,Li W,Liu F.Inhibition of proliferation and induction of apoptosis by genistein in colon cancer HT-29 cells[J].Cancer Lett,2004; 215(2)∶159.
7.	Bektic J,Guggenberger R,Eder IE,et al.Molecular effects of the isoflavonoid genistein in prostate cancer[J].Clin Prostate Cancer,2005; 4(2)∶124.
8.	Chou TC,Talalay P.Quantitative analysis of dose-effect relationships:the combined effects of multiple drugs or enzyme inhibitors[J].Adv Enzyme Regul,1984; 22∶27.
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10.	Shao ZM,Wu J,Shen ZZ,et al.Genistein exerts multiple suppressive effects on human breast carcinoma cells[J].Cancer Res,1998; 58(21)∶4851.
11.	Davis JN,Singh B,Bhuiyan M,et al.Genistein-induced upregulation of p21WAF1,downregulation of cyclin B,and induction of apoptosis in prostate cancer cells[J].Nutr Cancer,1998; 32(3)∶123.
12.	Yan C,Han R.Genistein suppresses adhesion-induced protein tyrosine phosphorylation and invasion of B16-BL6 melanoma cells[J].Cancer Lett,1998; 129(1)∶117.
13.	Arai N,Strom A,Rafter JJ,et al.Estrogen receptor beta mRNA in colon cancer cells:growth effects of estrogen and genistein[J].Biochem Biophys Res Commun,2000; 270(2)∶425.
14.	Ravindranath MH,Muthugounder S,Presser N,et al.Anticancer therapeutic potential of soy isoflavone,genistein[J].Adv Exp Med Biol,2004; 546∶121.
15.	Sarkar FH,Adsule S,Padhye S,et al.The role of genistein and synthetic derivatives of isoflavone in cancer prevention and therapy[J].Mini Rev Med Chem,2006; 6(4)∶401.
16.	金懋林.進展期胃癌全身化學治療新進展[J].外科理論與實踐,2003; 8(1)∶18.
17.	劉福坤,姚學權.胃癌的新輔助化療[J].中國普外基礎與臨床雜志,2006; 13(1)∶17.
18.	黃斌,陳志新.胃癌淋巴靶向化療的研究進展[J].中國普外基礎與臨床雜志,2006; 13(5)∶ 617.
19.	Hwang JT,Ha J,Park OJ.Combination of 5-fluorouracil and genistein induces apoptosis synergistically in chemo-resistant cancer cells through the modulation of AMPK and COX-2 signaling pathways[J].Biochem Biophys Res Commun,2005; 332(2)∶433.
20.	Banerjee S,Zhang Y,Wang Z,et al.In vitro and in vivo molecular evidence of genistein action in augmenting the efficacy of cisplatin in pancreatic cancer[J].Int J Cancer,2007; 120(4)∶906.
21.	Siwinska A,Opolski A,Chrobak A,et al.Potentiation of the antiproliferative effect in vitro of doxorubicin,cisplatin and genistein by new analogues of vitamin D[J].Anticancer Res,2001; 21(3B)∶1925.
22.	McDonnell CO,Holden G,Sheridan ME,et al.Improvement in efficacy of chemoradiotherapy by addition of an antiangiogenic agent in a murine tumor model[J].J Surg Res,2004; 116(1)∶19.
23.	Tamura S,Bito T,Ichihashi M,et al.Genistein enhances the cisplatin-induced inhibition of cell growth and apoptosis in human malignant melanoma cells[J].Pigment Cell Res,2003; 16(5)∶470.
24.	Evan GI,Vousden KH.Proliferation,cell cycle and apoptosis in cancer[J].Nature,2001; 411(6835)∶342.
25.	葉正寶,馬韜.胃腸道癌瘤的化學治療[A].見:朱正綱主編.胃腸道腫瘤外科綜合治療新技術[M].北京:人民軍醫出版社,2002∶154～180.

1. <><> [1]Barnes S.Effect of genistein on in vitro and in vivo models of cancer[J].J Nutr,1995; 125(3 Suppl)∶777S.
2. 龔軍,王正文,朱明才,等.金雀異黃素和5-FU對人結腸癌細胞株(SW480)的相互作用的體外觀察[J].中國現代醫學雜志,2006; 16(6)∶873.
3. 龔軍,王正文,朱明才,等.金雀異黃素和5-FU對人結腸癌細胞株(Colo320)的相互作用的體外觀察[J].中國醫師雜志,2006; 8(12)∶1601.
4. 張艷,田慶偉,李亞義.金雀異黃素對人乳癌細胞MCF-7增殖和凋亡的影響[J].天津醫科大學學報,2005; 11(1)∶42.
5. Shon YH,Park SD,Nam KS.Effective chemopreventive activity of genistein against human breast cancer cells[J].J Biochem Mol Biol,2006; 39(4)∶448.
6. Yu Z,Li W,Liu F.Inhibition of proliferation and induction of apoptosis by genistein in colon cancer HT-29 cells[J].Cancer Lett,2004; 215(2)∶159.
7. Bektic J,Guggenberger R,Eder IE,et al.Molecular effects of the isoflavonoid genistein in prostate cancer[J].Clin Prostate Cancer,2005; 4(2)∶124.
8. Chou TC,Talalay P.Quantitative analysis of dose-effect relationships:the combined effects of multiple drugs or enzyme inhibitors[J].Adv Enzyme Regul,1984; 22∶27.
9. 韓銳主編.抗癌藥物研究與實驗技術[M].北京:北京醫科大學中國協和醫科大學聯合出版社,1997∶399～400.
10. Shao ZM,Wu J,Shen ZZ,et al.Genistein exerts multiple suppressive effects on human breast carcinoma cells[J].Cancer Res,1998; 58(21)∶4851.
11. Davis JN,Singh B,Bhuiyan M,et al.Genistein-induced upregulation of p21WAF1,downregulation of cyclin B,and induction of apoptosis in prostate cancer cells[J].Nutr Cancer,1998; 32(3)∶123.
12. Yan C,Han R.Genistein suppresses adhesion-induced protein tyrosine phosphorylation and invasion of B16-BL6 melanoma cells[J].Cancer Lett,1998; 129(1)∶117.
13. Arai N,Strom A,Rafter JJ,et al.Estrogen receptor beta mRNA in colon cancer cells:growth effects of estrogen and genistein[J].Biochem Biophys Res Commun,2000; 270(2)∶425.
14. Ravindranath MH,Muthugounder S,Presser N,et al.Anticancer therapeutic potential of soy isoflavone,genistein[J].Adv Exp Med Biol,2004; 546∶121.
15. Sarkar FH,Adsule S,Padhye S,et al.The role of genistein and synthetic derivatives of isoflavone in cancer prevention and therapy[J].Mini Rev Med Chem,2006; 6(4)∶401.
16. 金懋林.進展期胃癌全身化學治療新進展[J].外科理論與實踐,2003; 8(1)∶18.
17. 劉福坤,姚學權.胃癌的新輔助化療[J].中國普外基礎與臨床雜志,2006; 13(1)∶17.
18. 黃斌,陳志新.胃癌淋巴靶向化療的研究進展[J].中國普外基礎與臨床雜志,2006; 13(5)∶ 617.
19. Hwang JT,Ha J,Park OJ.Combination of 5-fluorouracil and genistein induces apoptosis synergistically in chemo-resistant cancer cells through the modulation of AMPK and COX-2 signaling pathways[J].Biochem Biophys Res Commun,2005; 332(2)∶433.
20. Banerjee S,Zhang Y,Wang Z,et al.In vitro and in vivo molecular evidence of genistein action in augmenting the efficacy of cisplatin in pancreatic cancer[J].Int J Cancer,2007; 120(4)∶906.
21. Siwinska A,Opolski A,Chrobak A,et al.Potentiation of the antiproliferative effect in vitro of doxorubicin,cisplatin and genistein by new analogues of vitamin D[J].Anticancer Res,2001; 21(3B)∶1925.
22. McDonnell CO,Holden G,Sheridan ME,et al.Improvement in efficacy of chemoradiotherapy by addition of an antiangiogenic agent in a murine tumor model[J].J Surg Res,2004; 116(1)∶19.
23. Tamura S,Bito T,Ichihashi M,et al.Genistein enhances the cisplatin-induced inhibition of cell growth and apoptosis in human malignant melanoma cells[J].Pigment Cell Res,2003; 16(5)∶470.
24. Evan GI,Vousden KH.Proliferation,cell cycle and apoptosis in cancer[J].Nature,2001; 411(6835)∶342.
25. 葉正寶,馬韜.胃腸道癌瘤的化學治療[A].見:朱正綱主編.胃腸道腫瘤外科綜合治療新技術[M].北京:人民軍醫出版社,2002∶154～180.

《中國普外基礎與臨床雜志》

金雀異黃素與5-氟尿嘧啶對SGC-7901 細胞的抑制作用

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

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《中國普外基礎與臨床雜志》

金雀異黃素與5-氟尿嘧啶對SGC-7901 細胞的抑制作用

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

Format

Content

摘要全文圖表視頻參考文獻施引文獻補充材料