PTEN、Fas/FasL和MMP-2在胃癌組織的表達及其臨床意義_《中國普外基礎與臨床雜志》

作者：

王鵬 , 郭琳 , 王強

中國醫科大學附屬盛京醫院胃腸外科（沈陽 110004）;

關鍵詞：

第10號染色體同源缺失性磷酸酶-張力蛋白 Fas/FasL系統基質金屬蛋白酶-2 胃癌免疫組織化學

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目的探討胃癌組織中第10號染色體同源缺失性磷酸酶-張力蛋白（PTEN）、Fas/FasL和基質金屬蛋白酶-2（MMP-2）的表達及其與胃癌生物學行為的關系。方法選擇臨床及病理資料齊全的胃癌蠟塊標本75例，另取正常胃黏膜組織15例作對照，采用SP免疫組化方法檢測PTEN、Fas/FasL和MMP-2在其中的表達。采用χ2檢驗和相關分析作統計學分析。結果 PTEN、Fas/FasL及MMP-2的表達與胃癌的浸潤深度、淋巴結轉移、TNM分期及腫瘤的組織分化程度均有關（P lt;0.05）。PTEN和Fas/FasL的表達呈正相關（r=0.401，P lt;0.001），MMP-2與Fas/FasL的表達呈負相關（r=－0.720，P lt;0.001），MMP-2與PTEN的表達呈負相關（r=－0.336，P lt;0.001）。結論胃癌組織中PTEN、Fas/FasL及MMP-2的表達陽性率可以成為胃癌診斷和預后的判斷指標。

引用本文： 王鵬,郭琳,王強. PTEN、Fas/FasL和MMP-2在胃癌組織的表達及其臨床意義. 中國普外基礎與臨床雜志, 2009, 16(12): 991-995. doi: 復制

1.	Li J, Yen C, Liaw D, et al. PTEN, a putative protein tyrosine phosphatase gene mutated in human brain, breast, and prostate cancer [J]. Science, 1997； 275(5308): 1943-1947.
2.	Li DM, Sun H. TEP1, encoded by a candidate tumor suppressor locus, is a novel protein tyrosine phosphatase regulated by transforming growth factor beta [J]. Cancer Res, 1997； 57 (11): 2124-2129.
3.	Steck PA, Pershouse MA, Jasser SA, et al. Identification of a candidate tumor suppressor gene MMAC1, at chromosome 10q23.3 that is mutated in multiple advanced cancers [J]. Nat Genet, 1997； 15(4): 356-362.
4.	熊敏, 吳一龍主編. 現代肺癌病理與臨床 [M]. 北京：北京科學出版社，2003: 97-98.
5.	Zhao MD, Hu XM, Sun DJ, et al. Expression of some tumor associated factors in human carcinogenesis and development of gastric carcinoma [J]. World J Gastroenterol, 2005； 11 (21): 3217-3221.
6.	Forsyth PA, Wong H, Laing TD, et al. Gelatinase-A (MMP-2), gelatinase-B (MMP-9) and membrane type matrix metalloproteinase-1 (MT1-MMP) are involved in different aspects of the pathophysiology of malignant gliomas [J]. Br J Cancer, 1999； 79 (11-12): 1828-1835.
7.	Yoo CH, Noh SH, Kim YI, et al. Comparison of prognostic significance of nodal staging between old (4th edition) and new (5th edition) UICC TNM classification for gastric carcinoma. International Union Against Cancer [J]. World J Surg, 1999; 23(5): 492-497.
8.	Jawhari A, Jordan S, Poole S, et al. Abnormal immunoreactivity of the E-cadherin-catenin complex in gastric carcinoma: relationship with patient survival [J]. Gastroenterology， 1997; 112(1): 46-54.
9.	陳昊, 李玉民, 何明彥, 等. 胃不典型增生和癌組織中Ki67和PTEN蛋白的表達及意義 [J]. 中國普外基礎與臨床雜志， 2007； 14（3）： 304-307.
10.	Tamura M, Gu J, Takino T, et al. Tumor suppressor PTEN inhibition of cell invasion, migration and growth: differential involvement of focal adhesion kinase and pl30Cas [J]. Cancer Res, 1999； 59(2): 442-449.
11.	Yang L, Kuang LG, Zheng HC, et al. PTEN encoding product: a marker for tumorigenesis and progression of gastric carcinoma [J]. World J Gastroenterol, 2003； 9(1): 35-39.
12.	Fei G, Ebert MP, Mawrin C, et al. Reduced PTEN expression in gastric cancer and in the gastric mucosa of gastric cancer relatives [J]. Eur J Gastroenterol Hepatol, 2002； 14(3): 297-303.
13.	李海民, 竇科峰, 李韌, 等. 肝癌組織中Fas和FasL mRNA表達水平的變化及其意義 [J]. 中國普外基礎與臨床雜志， 2005； 12（3）： 284-286.
14.	何貴金, 于發強, 高沁怡, 等. 103鈀放射性支架對Fas基因表達影響及與膽管癌細胞凋亡關系研究 [J]. 中國普外基礎與臨床雜志， 2007； 14（3）： 284-286.
15.	Kusakabe T, Nashimoto A, Honma K, et al. Fatty acid synthase is highly expressed in carcinoma, adenoma and in regenerative epithelium and intestinal metaplasia of the stomach [J]. Histopathology, 2002； 40(1): 71-79.
16.	Morimoto Y, Hizuta A, Ding EX, et al. Funtional expression of Fas and Fas ligand on human intestinal intraepithelial lymphocytes [J]. Clin Exp immuno1, 1999； 116(1): 84-89.
17.	項良光, 林新龍, 偉青, 等. 耐阿霉素肝癌細胞HepG2表達Fas的變化 [J]. 中國普外基礎與臨床雜志， 2000； 7（4）： 229-230.
18.	Elnemr A, Ohta T, Yachie A, et al. Human pancreatic cancer cells disable function of Fas receptors at several levels in Fas signal transduction pathway [J]. Int J Oncol, 2001； 18(2): 311-316.
19.	Nabeshima K, Inoue T, Shimao Y, et al． Front-cells- specific expression of membrane-type 1 matrix metalloproteinase and gelatinase A during cohort migration of colon carcinoma cells induced by hepatocyte growth factor／scatter factor [J]． Cancer Res, 2000； 60(13): 3364-3369.
20.	Grigioni WF, D’Errico A, Fiorentino M, et al. Gelatinase A (MMP-2) and its mRNA detected in both neoplastic and stromal cells of tumors with different invasive and metastatic properties [J]． Diagn Mol Pathol, 1994； 3(3): 163-169.
21.	Nomura H, Fujimoto N, Seiki M, et al. Enhanced production of matrix metalloproteinases and activation of matrix metalloproteinase 2 (gelatinase A) in human gastric carcinomas [J]. Int J Cancer, 1996； 69(1): 9-16.
22.	Ko BK, Cho HR, Choi DW, et al. Reduced expression of tissue inhibitor of metalloproteinase in nodal metastasis of stomach cancer [J]. J Korean Med Sci, 1998； 13 (3)： 286-290.
23.	Otani Y. The collagenase activities, interstitial collagenase and type Ⅳ collagenase, in human stomach cancer: with special reference to local spreading and lymph node metastasis [J]. Keio J Med, 1990； 39(3): l59-167.
24.	Ko K, Yazumi S, Yoshikawa K, et al. Activation of fibroblast-derived matrix metalloproteinase-2 by colon-cancer cells in non-contact Co-cultures [J]. Int J Cancer, 2000； 87(2): 165-171.
25.	劉偉, 安杰, 張超. 尼美舒利對結腸腺癌細胞MMP-2表達的影響 [J]. 中國普外基礎與臨床雜志， 2005； 12（6）： 585-588.

1. Li J, Yen C, Liaw D, et al. PTEN, a putative protein tyrosine phosphatase gene mutated in human brain, breast, and prostate cancer [J]. Science, 1997； 275(5308): 1943-1947.
2. Li DM, Sun H. TEP1, encoded by a candidate tumor suppressor locus, is a novel protein tyrosine phosphatase regulated by transforming growth factor beta [J]. Cancer Res, 1997； 57 (11): 2124-2129.
3. Steck PA, Pershouse MA, Jasser SA, et al. Identification of a candidate tumor suppressor gene MMAC1, at chromosome 10q23.3 that is mutated in multiple advanced cancers [J]. Nat Genet, 1997； 15(4): 356-362.
4. 熊敏, 吳一龍主編. 現代肺癌病理與臨床 [M]. 北京：北京科學出版社，2003: 97-98.
5. Zhao MD, Hu XM, Sun DJ, et al. Expression of some tumor associated factors in human carcinogenesis and development of gastric carcinoma [J]. World J Gastroenterol, 2005； 11 (21): 3217-3221.
6. Forsyth PA, Wong H, Laing TD, et al. Gelatinase-A (MMP-2), gelatinase-B (MMP-9) and membrane type matrix metalloproteinase-1 (MT1-MMP) are involved in different aspects of the pathophysiology of malignant gliomas [J]. Br J Cancer, 1999； 79 (11-12): 1828-1835.
7. Yoo CH, Noh SH, Kim YI, et al. Comparison of prognostic significance of nodal staging between old (4th edition) and new (5th edition) UICC TNM classification for gastric carcinoma. International Union Against Cancer [J]. World J Surg, 1999; 23(5): 492-497.
8. Jawhari A, Jordan S, Poole S, et al. Abnormal immunoreactivity of the E-cadherin-catenin complex in gastric carcinoma: relationship with patient survival [J]. Gastroenterology， 1997; 112(1): 46-54.
9. 陳昊, 李玉民, 何明彥, 等. 胃不典型增生和癌組織中Ki67和PTEN蛋白的表達及意義 [J]. 中國普外基礎與臨床雜志， 2007； 14（3）： 304-307.
10. Tamura M, Gu J, Takino T, et al. Tumor suppressor PTEN inhibition of cell invasion, migration and growth: differential involvement of focal adhesion kinase and pl30Cas [J]. Cancer Res, 1999； 59(2): 442-449.
11. Yang L, Kuang LG, Zheng HC, et al. PTEN encoding product: a marker for tumorigenesis and progression of gastric carcinoma [J]. World J Gastroenterol, 2003； 9(1): 35-39.
12. Fei G, Ebert MP, Mawrin C, et al. Reduced PTEN expression in gastric cancer and in the gastric mucosa of gastric cancer relatives [J]. Eur J Gastroenterol Hepatol, 2002； 14(3): 297-303.
13. 李海民, 竇科峰, 李韌, 等. 肝癌組織中Fas和FasL mRNA表達水平的變化及其意義 [J]. 中國普外基礎與臨床雜志， 2005； 12（3）： 284-286.
14. 何貴金, 于發強, 高沁怡, 等. 103鈀放射性支架對Fas基因表達影響及與膽管癌細胞凋亡關系研究 [J]. 中國普外基礎與臨床雜志， 2007； 14（3）： 284-286.
15. Kusakabe T, Nashimoto A, Honma K, et al. Fatty acid synthase is highly expressed in carcinoma, adenoma and in regenerative epithelium and intestinal metaplasia of the stomach [J]. Histopathology, 2002； 40(1): 71-79.
16. Morimoto Y, Hizuta A, Ding EX, et al. Funtional expression of Fas and Fas ligand on human intestinal intraepithelial lymphocytes [J]. Clin Exp immuno1, 1999； 116(1): 84-89.
17. 項良光, 林新龍, 偉青, 等. 耐阿霉素肝癌細胞HepG2表達Fas的變化 [J]. 中國普外基礎與臨床雜志， 2000； 7（4）： 229-230.
18. Elnemr A, Ohta T, Yachie A, et al. Human pancreatic cancer cells disable function of Fas receptors at several levels in Fas signal transduction pathway [J]. Int J Oncol, 2001； 18(2): 311-316.
19. Nabeshima K, Inoue T, Shimao Y, et al． Front-cells- specific expression of membrane-type 1 matrix metalloproteinase and gelatinase A during cohort migration of colon carcinoma cells induced by hepatocyte growth factor／scatter factor [J]． Cancer Res, 2000； 60(13): 3364-3369.
20. Grigioni WF, D’Errico A, Fiorentino M, et al. Gelatinase A (MMP-2) and its mRNA detected in both neoplastic and stromal cells of tumors with different invasive and metastatic properties [J]． Diagn Mol Pathol, 1994； 3(3): 163-169.
21. Nomura H, Fujimoto N, Seiki M, et al. Enhanced production of matrix metalloproteinases and activation of matrix metalloproteinase 2 (gelatinase A) in human gastric carcinomas [J]. Int J Cancer, 1996； 69(1): 9-16.
22. Ko BK, Cho HR, Choi DW, et al. Reduced expression of tissue inhibitor of metalloproteinase in nodal metastasis of stomach cancer [J]. J Korean Med Sci, 1998； 13 (3)： 286-290.
23. Otani Y. The collagenase activities, interstitial collagenase and type Ⅳ collagenase, in human stomach cancer: with special reference to local spreading and lymph node metastasis [J]. Keio J Med, 1990； 39(3): l59-167.
24. Ko K, Yazumi S, Yoshikawa K, et al. Activation of fibroblast-derived matrix metalloproteinase-2 by colon-cancer cells in non-contact Co-cultures [J]. Int J Cancer, 2000； 87(2): 165-171.
25. 劉偉, 安杰, 張超. 尼美舒利對結腸腺癌細胞MMP-2表達的影響 [J]. 中國普外基礎與臨床雜志， 2005； 12（6）： 585-588.

《中國普外基礎與臨床雜志》

PTEN、Fas/FasL和MMP-2在胃癌組織的表達及其臨床意義

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《中國普外基礎與臨床雜志》

PTEN、Fas/FasL和MMP-2在胃癌組織的表達及其臨床意義

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

Format

Content

摘要全文圖表視頻參考文獻施引文獻補充材料