聯合檢測外周血hTERT mRNA及GGT mRNA-H表達對小肝癌早期診斷的價值_《中國普外基礎與臨床雜志》

作者：

 隋璐璐 ¹ , 劉小方 ² , 許政 ²

1.青島大學醫學院（山東青島 266071）;;
2.青島大學醫學院附屬煙臺毓璜頂醫院肝膽胰外科（山東煙臺 264000）;

關鍵詞：

肝細胞肝癌端粒酶端粒酶逆轉錄酶谷氨酰胺轉移酶聚合酶鏈反應腫瘤

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目的研究hTERT mRNA 及 GGT mRNA-H 在小肝癌患者外周血中的表達,并探討其對肝癌早期診斷的價值。方法采用RT-PCR方法檢測30例小肝癌患者、18例肝炎肝硬變患者以及12例正常受試者外周血hTERT mRNA及GGT mRNAH的表達。結果小肝癌組患者hTERT mRNA及GGT mRNAH表達陽性率分別為80.0%（24/30）和46.7%（14/30），均高于AFP的檢測陽性率(26.7%,8/30)，P lt;0.05。正常對照組均未檢測到hTERT mRNA和GGT mRNAH的表達。肝炎肝硬變組患者hTERT mRNA的表達陽性率為33.3%（6/18），未檢測到GGT mRNAH的表達。聯合檢測小肝癌患者hTERT mRNA及GGT mRNA-H表達的陽性率明顯高于AFP的陽性率（86.7%比26.7%），P lt;0.05。結論hTERT mRNA及GGT mRNA-H在小肝癌患者中特異性表達，聯合檢測外周血hTERT mRNA及GGT mRNAH 可提高小肝癌的早期診斷率。

引用本文： 隋璐璐,劉小方,許政. 聯合檢測外周血hTERT mRNA及GGT mRNA-H表達對小肝癌早期診斷的價值. 中國普外基礎與臨床雜志, 2011, 18(1): 59-62. doi: 復制

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12.	Gigek CO, Leal MF, Silva PN, et al. hTERT methylation and expression in gastric cancer [J]. Biomarkers, 2009, 14(8): 630636.
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14.	Pasrija T, Srinivasan R, Behera D, et al. Telomerase activity in sputum and telomerase and its components in biopsies of advanced lung cancer [J]. Eur J Cancer, 2007, 43(9): 14761482.
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19.	Hashimoto Y, Murakami Y, Uemura K, et al. Detection of human telomerase reverse transcriptase (hTERT) expression in tissue and pancreatic juice from pancreatic cancer [J]. Surgery, 2008, 143(1): 113125.
20.	Bravaccini S, Casadio V, Amadori D, et al. The current role of telomerase in the diagnosis of bladder cancer [J]. Indian J Urol, 2009, 25(1): 4046.
21.	Varela E, Blasco MA. 2009 nobel prize in physiology or medicine: telomeres and telomerase [J]. Oncogene, 2010, 29(11): 15611565.
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23.	Han GQ, Qin CY, Shu RH. The analysis of γglutamyl transpeptidase gene in different type liver tissues [J]. World J Gastroenterol, 2003, 9(2): 276280.
24.	Tsutsumi M, Sakamuro D, Takada A, et al. Detection of a unique γglutamyl transpeptidase messenger RNA species closely related to the development of hepatocellular carcinoma in humans: a new candidate for early diagnosis of hepatocellular carcinoma [J]. Hepatology, 1996, 23(5): 10931097.
25.	Daubeuf S, Accaoui MJ, Pettersen I, et al. Differential regulation of γglutamyltransferase mRNAs in four human tumour cell lines [J]. Biochim Biophys Acta, 2001, 1568(1): 6773.
26.	徐守偉, 劉曉華, 韓國慶, 等. 原發性肝癌患者外周血、肝組織中GGT mRNAH 表達及意義 [J]. 山東醫藥, 2009, 49(7): 6061.

1. Llovet JM, Bruix J. Novel advancements in the management of hepatocellular carcinoma in 2008 [J]. J Hepatol, 2008, 48 Suppl 1: S20S37.
2. Zhang Y, Li Q, Liu N, et al. Detection of MAGE1, MAGE3 and AFP mRNA as multimarker by realtime quantitative PCR assay: a possible predictor of hematogenous micrometastasis of hepatocellular carcinoma [J]. Hepatogastroenterology, 2008, 55(88): 22002206.
3. 錢林學, 劉玉江, 丁惠國. 小肝癌的影像學診斷進展 [J]. 世界華人消化雜志, 2010, 18(5): 479486.
4. 陳孝平, 張萬廣. 原發性肝癌面臨的難點與對策 [J]. 中國普外基礎與臨床雜志， 2008, 15(4): 231233.
5. 沈嘯洪, 李慧鍇, 張倜. 肝癌外科規范化治療若干問題探討 [J]. 中國普外基礎與臨床雜志, 2009, 16(4): 261264.
6. Miura N, Osaki Y, Nagashima M, et al. A novel biomarker TERTmRNA is applicable for early detection of hepatoma [J]. BMC Gastroenterol, 2010, 10: 4657.
7. Wang NY, Zhang D, Zhao W, et al. Clinical application of an enzymelinked immunosorbent assay detecting hepatomaspecific γglutamyltransferase [J]. Hepatol Res, 2009, 39(10): 979987.
8. 中華醫學會傳染病與寄生蟲病學分會、肝病學分會. 病毒性肝炎防治方案 [J]. 中華肝臟病雜志, 2000, 8(6): 324329.
9. ElSerag HB, Rudolph KL. Hepatocellular carcinoma: epidemiology and molecular carcinogenesis [J]. Gastroenterology, 2007, 132(7): 25572576.
10. Liu H, Li P, Zhai Y, et al. Diagnostic value of glypican3 in serum and liver for primary hepatocellular carcinoma [J]. World J Gastroenterol, 2010, 16(35): 44104415.
11. Terrin L, Rampazzo E, Pucciarelli S. et al. Relationship between tumor and plasma levels of hTERT mRNA in patients with colorectal cancer: implications for monitoring of neoplastic disease [J]. Clin Cancer Res, 2008, 14(22): 74447451.
12. Gigek CO, Leal MF, Silva PN, et al. hTERT methylation and expression in gastric cancer [J]. Biomarkers, 2009, 14(8): 630636.
13. Udomchaiprasertkul W, Narong S, Kongsema M, et al. Detection of hTERT mRNA in gastrointestinal tract cancer specimens [J]. Southeast Asian J Trop Med Public Health, 2008, 39(2): 324327.
14. Pasrija T, Srinivasan R, Behera D, et al. Telomerase activity in sputum and telomerase and its components in biopsies of advanced lung cancer [J]. Eur J Cancer, 2007, 43(9): 14761482.
15. Capezzone M, Marchisotta S, Cantara S, et al. Telomeres and thyroid cancer [J]. Curr Genomics, 2009, 10(8): 526533.
16. Perona R, MachadoPinilla R, Manguan C, et al. Telomerase deficiency and cancer susceptibility syndromes [J]. Clin Transl Oncol, 2009, 11(11): 711714.
17. Stewart SA, Bertuch AA. The role of telomeres and telomerase in cancer research [J]. Cancer Res, 2010, 70(19): 73657371.
18. Shay JW, Wright WE. Telomeres and telomerase in normal and cancer stem cells [J]. FEBS Lett, 2010, 584(17): 38193825.
19. Hashimoto Y, Murakami Y, Uemura K, et al. Detection of human telomerase reverse transcriptase (hTERT) expression in tissue and pancreatic juice from pancreatic cancer [J]. Surgery, 2008, 143(1): 113125.
20. Bravaccini S, Casadio V, Amadori D, et al. The current role of telomerase in the diagnosis of bladder cancer [J]. Indian J Urol, 2009, 25(1): 4046.
21. Varela E, Blasco MA. 2009 nobel prize in physiology or medicine: telomeres and telomerase [J]. Oncogene, 2010, 29(11): 15611565.
22. Theimer CA, Feigon J. Structure and function of telomerase RNA [J]. Curr Opin Struct Biol, 2006, 16(3): 307318.
23. Han GQ, Qin CY, Shu RH. The analysis of γglutamyl transpeptidase gene in different type liver tissues [J]. World J Gastroenterol, 2003, 9(2): 276280.
24. Tsutsumi M, Sakamuro D, Takada A, et al. Detection of a unique γglutamyl transpeptidase messenger RNA species closely related to the development of hepatocellular carcinoma in humans: a new candidate for early diagnosis of hepatocellular carcinoma [J]. Hepatology, 1996, 23(5): 10931097.
25. Daubeuf S, Accaoui MJ, Pettersen I, et al. Differential regulation of γglutamyltransferase mRNAs in four human tumour cell lines [J]. Biochim Biophys Acta, 2001, 1568(1): 6773.
26. 徐守偉, 劉曉華, 韓國慶, 等. 原發性肝癌患者外周血、肝組織中GGT mRNAH 表達及意義 [J]. 山東醫藥, 2009, 49(7): 6061.

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聯合檢測外周血hTERT mRNA及GGT mRNA-H表達對小肝癌早期診斷的價值

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《中國普外基礎與臨床雜志》

聯合檢測外周血hTERT mRNA及GGT mRNA-H表達對小肝癌早期診斷的價值

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