Effect of Ginsenoside Rg1 on the Survival Rate andNeuronal Plasticity of Primary Cultured Cortical Neurons_West China Medical Journal

Authors：

PENG Tao ¹ , Yinghui ² , CHEN Shaohong ¹ , et al. ¹

1The Attached Hospital of Chengdu University of Traditional Chinese Medicine,Chengdu 610075,China;2School of Preclinical and Forensic Medicine in Sichuan University,Chengdu 610041,China;

Corresponding?author：

Keywords：

人參皂甙Rg1; 神經元; 神經可塑性; GAP 43; NF 200

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目的：研究人參皂甙Rg 1對原代培養胎鼠腦神經細胞存活和可塑性的影響。方法：實驗分為：實驗組（人參皂甙Rg 1 1 mg/L,10 mg/L,100 mg/L），陽性藥物對照組（bFGF 20 μg/L）以及空白對照組。相差倒置顯微鏡觀察細胞生長情況，并測量細胞突起的長度；用MTT法測定培養細胞的存活率；Western-blot法檢測神經生長相關蛋白GAP-43和神經絲蛋白NF-200的表達。結果：（1）細胞平均突起長度：實驗高中劑量組神經元突起的平均長度均長于對照組。（2）MTT值：實驗高中低劑量組的灰度均明顯大于對照組。（3）GAP43和NF 200的表達：實驗高中劑量組的蛋白表達均明顯大于對照組。結論：人參皂甙Rg 1對于體外培養的胎鼠腦神經細胞的存活有較強的維持作用，并能促進突起生長，使神經可塑性相關蛋白表達上調。

Citation： PENG Tao,Yinghui,CHEN Shaohong,et al.. Effect of Ginsenoside Rg1 on the Survival Rate andNeuronal Plasticity of Primary Cultured Cortical Neurons. West China Medical Journal, 2008, 23(1): 106-107. doi: Copy

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1. 劉.人參皂甙Rg1抗衰老和促智作用及其機制研究[J].生理科學進展，1996，27（2）：139－142..
2. 李會娟,樊均明.人參皂甙Rgl的藥理作用及機制[J].四川醫學,2006,27:1007-1008..
3. 劉穎,李璽,袁海峰.人參皂甙Rg1促進學習記憶作用研究進展[J].中國中西醫結合雜志,2006,26(10):956-960..
4. D.L.斯佩克特,R.D.戈德曼,L.A.萊因萬德.細胞實驗指南[M].北京:科學出版社,2001:62-65..
5. 郭琴,毛萌,羅小麗,等.神經元原代培養方法的改進[J].華西醫學,2007,22(3):566-568..
6. Tao Peng,Yinghui Liu,Chunlei Yang,et al.A New Peptide with Membrane-permeable Function Derived from Human Circadian Proteins[J].Acta Biochim Biophys Sin,2004,36(9):629-636..
7. 溫進坤,韓梅.醫學分子生物學理論與研究技術[M].北京:科學出版社,2002:225-232..
8. Skene JHP.Axonal-growth associated proteins[J].Annu Rev Neurosci,1989，12:127-156..
9. Julien JP,Mushynski WE.Neurofilaments in health and disease[J].Prog Nucleic Acid Res Mol Biol,1998,61:1-23..
10. Cheng B,Mattson MP.NGF and bFGF protect rat hippocampal and human cortical neurons against hypoglycemic damage by stabilizing calcium homeostasis[J].Neuron,1991,7:1031-1041..
11. Zagrebelsky M,Buffo A,Skerra A,et al.retrograde regulationof growth-associated gene expression in adult rat Purkinje cells by myelin-associated neurite growth inhibitory proteins[J].The Journal of Neuroscience,1998,18(19):7912-7929.

West China Medical Journal

Effect of Ginsenoside Rg1 on the Survival Rate andNeuronal Plasticity of Primary Cultured Cortical Neurons

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