結締組織病患者結膜印跡細胞凋亡研究_《中國循證醫學雜志》

作者：

1. 天津醫科大學第二醫院眼科(天津 300211);2. 山西醫科大學第一臨床醫學院眼科(太原 030001);

關鍵詞：

印跡細胞學凋亡干眼結締組織病

DOI：

10.7507/1672-2531.20090168

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目的　探討結膜印跡細胞的流式細胞術凋亡檢測在干眼發病機制研究與結締組織病患者干眼診斷中的應用價值。
方法　對結締組織病患者 60 例(120 眼)進行眼科病史詢問、淚液分泌實驗I(S-I-T)、淚膜破裂時間(BUT)及熒光素染色計分(FL)檢查,根據檢查結果和有無 Sj?gren 綜合征分為非干燥綜合征無干眼(NSS1)組、非干燥綜合征干眼(NSS2)組、干燥綜合征無干眼(SS1)組和干燥綜合征干眼(SS2)組。并對其行結膜印跡細胞流式細胞術凋亡檢測。
結果　結膜上皮細胞凋亡百分比除 NSS1 組與 SS1 組間差異無統計學意義(P=0.998)外,其余各組差異均有統計學意義(P lt;0.001)。且細胞凋亡百分比與 FL 成正相關(r=0.926,P lt;0.001),與 S-I-T、BUT 結果成負相關(r= –0.712,r= –0.818,P lt;0.001)。有無干眼和有無干燥均影響結膜上皮細胞凋亡量,且干眼與干燥有交互作用。
結論　細胞凋亡可能是造成干眼眼表損害的重要因素,凋亡檢測有助于結締組織病患者干眼的診斷。干眼和干燥均可使結膜上皮細胞凋亡增加。印跡細胞流式細胞凋亡檢測是一項微創、有效的眼表凋亡檢測方法。

引用本文： 黃冠南,王麗聰,張虹. 結締組織病患者結膜印跡細胞凋亡研究. 中國循證醫學雜志, 2009, 09(9): 949-952. doi: 10.7507/1672-2531.20090168 復制

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1. The definition and classification of dry eye disease: report of the Definition and Classification Subcommittee of the International Dry Eye WorkShop (2007). Ocul Surf, 2007, 5(2): 75-92.
2. Steven Y, Song XJ, Farley W, et al. Apoptosis of Ocular Surface Cells in Experimentally Induced Dry Eye. Invest Ophthalmol Vis Sci, 2003, 44: 124-129.
3. Afonso AA, Monroy D, Stern ME, et al. Correlation of tear fluorescein clearance and Schirmer test scores with ocular irritation symptoms. Ophthalmology, 1999, 106: 803-810.
4. 劉祖國. 主編. 眼表疾病學. 第1版. 北京: 人民衛生出版社, 2003. 293.
5. 柯寧, 趙敏. 干眼的免疫學研究現狀及進展. 中國實用眼科雜志, 2006, 24(10): 1009-1013.
6. Pflugfelder SC, Huang AJ, Feuer W, et al. Conjunctival cytologic features of primary Sj?gren syndrome. Ophthalmology, 1990, 97(8): 985-991.
7. Pflugfelder SC, Jones D, Ji Z, et a1. Altered cytokine balance in the tear fluid and conjunctiva of patients with Sj?gren’s syndrome keratoconjunctivitis sicca. Curr Eye Res, 1999, 19: 201-211.
8. Gao J, Schwal TA, Addeo JV, et a1. The role of apoptosis in the pathogenesis of canine keratoconjunctivitis sicca: the effect of topical cyclosporin A therapy. Cornea, 1998, 17(6): 654-663.
9. Tsubota K, Fujita H, Tadano K, et al. Abnormal expression and function of Fas ligand of lachrymal glands and peripheral blood in Sj?gren’s syndrome patients with enlarged exocrine glands. Clin Exp Immunol, 2002, 129: 177-182.
10. Brignole F, Pissella PJ, Goldschild M, et a1. Flow cytometric analysis of inflammatory markers in conjunctival epithelial cells of patients with dry eye. Invest Ophthalmol Vis Sci, 2000, 41: 1356-1363.
11. 肖啟國, 劉祖國, 張梅. 多西環素對培養的人結膜上皮細胞炎性反應及凋亡的影響. 中華眼科雜志, 2005, 41(9): 842-845.
12. Sahai A, Malik P. Dry eye: prevalence and attributable risk factors in a hospital-based population. Indian J Ophthalmol, 2005, 53(2): 87-91.
13. Vitali C, Bombardieri S, Jonsson R, et al. Classification criteria for Sj?gren’s syndrome: a revised version of the European criteria proposed by the American-European Consensus Group. Ann Rheum Dis, 2002, 61(6): 554-558.

《中國循證醫學雜志》

結締組織病患者結膜印跡細胞凋亡研究

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《中國循證醫學雜志》

結締組織病患者結膜印跡細胞凋亡研究

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