HIV/AIDS實驗室檢測及其研究進展_《中國循證醫學雜志》

作者：

 白浪 , 雷秉鈞

四川大學華西醫院感染性疾病中心（成都 610041）;

關鍵詞：

獲得性免疫缺陷綜合征人類免疫缺陷病毒實驗室檢測

DOI：

10.7507/1672-2531.20080045

視頻：

導出 下載 收藏 掃碼 引用

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

獲得性免疫缺陷綜合征（AIDS）是由人類免疫缺陷病毒（HIV）引起的一種嚴重危脅人類健康的傳染病。實驗室檢測是診斷HIV/AIDS的主要依據。HIV實驗室檢測一般包括病毒抗體和抗原檢測、病毒核酸檢測、免疫細胞檢測及病毒耐藥檢測。20多年來，HIV的實驗室診斷方法取得很大進展，特別是近年來免疫學方法和分子生物學方法的應用為臨床診治和流行病學調查提供了有力的幫助。對HIV的檢測，靈敏度及特異度高的分析方法將有助于HIV的早期診斷、避免漏檢，也有助于對抗艾滋病藥物的療效評價、預測和監測疾病進程，提高檢測的靈敏性，縮短檢測的窗口期，是HIV診斷方法和診斷試劑持續發展的主要方向。隨著對HIV/AIDS研究的不斷深入及分子生物學技術的飛速發展，各種檢測HIV的新技術層出不窮，HIV的實驗室檢測正朝著簡便、快速、敏感、準確及自動化方向發展。

引用本文： 白浪,雷秉鈞. HIV/AIDS實驗室檢測及其研究進展. 中國循證醫學雜志, 2008, 08(3): 206-209. doi: 10.7507/1672-2531.20080045 復制

1.	Mylonakis E, Paliou M, Lally M, et al. Laboratory testing for infection with the human immunodeficiency virus:established and novel approaches. Am J Med, 2000, 109(7): 568-576.
2.	Gurtler L, Muhlbacher A, Michl U, et al. Reduction of the diagnostic window with a new combined p24 antigen and human immunodeficiency virus antibody screening assay. J Virol Methods, 1998, 75(1): 27-38.
3.	Weber B, Berger A, Rabenau H, et al. Evaluation of a New Combined Antigen and Antibody Human Immunodeficiency Virus Screening Assay ,VIDAS HIV DUO Ultra. J Clin Microbiol, 2002, 40(4): 1420-1426.
4.	Weber B, Gürtler L, Thorstensson R, et al. Multicenter Evaluation of a New Automated Fourth-generation Human Immunodeficiency Virus Screening Assay with a sensitive Antigen Detection Module and High Specificity. J Clin Microbiol, 2002, 40(6): 1938-1946.
5.	Gurtler LG, Eberle J, Lorbeer B, et al. Sensitivity and specificity of commercial ELISA kits for screening anti-LAV/HTLV III. J Virol Methods, 1987, 15(1): 11-23.
6.	Petricciani JC, Epstein JS. The effects of the AIDS epidemic on the safety of the nation’s blood supply. Public Health Rep, 1988, 103(3): 236-241.
7.	Barbe F, Klein M, Badonnel Y. Early detection of antibodies to human immunodeficiency virus 1 by a third-generation enzyme immunoassay.A comparative study with the results of second-generation immunoassays and western blot. Ann Biol Clin(Paris), 1994, 52(5): 341-345.
8.	Suligoi B, Galli C, Massi M, et al. Precision and Accuracy of a Procedure for Detecting Recent Human Immunodeficiency Virus Infections by Calculating the Antibody Avidity Index by an Automated Immunoassay-Based Method. J Clin. Microbiol, 2002, 40(11): 4015-4020.
9.	Ly TD, Laperche S, Brennan C, et al. Evaluation of the sensitivity and specificity of six HIV combined p24 antigen and antibody assays. J Virol Methods, 2004, 122(2): 185-194.
10.	Bernard M Branson, MD. Rapid Test for HIV Antiboby. AIDS Reviews, 2000, 2: 76-83.
11.	魏經新. 免疫層析試驗檢測抗HIV1+2的臨床應用價值. 中國艾滋病性病, 2003, 9(5): 280-281.
12.	鄭曉麗. HIV檢測研究進展與發展趨勢. 現代檢驗醫學雜志, 2004, 19(6): 64-67.
13.	馮曉英, 徐曉琴. 吸毒人群尿液血液標本HIV-1抗體ELISA檢測對比分析. 中國性病艾滋病, 2003, 9(3): 132-133.
14.	趙立慶, 雷素萍, 李連學, 等. ELISA法檢測尿液中HIV-1抗體的研究分析. 中國艾滋病性病, 2003, 9(5): 276-277.
15.	Nguyen KA, Busch MP. Evolving strategies for diagnosing human immunodeficiency virus infection. Am J Med, 2000, 109(7): 595-597.
16.	Celum CL, Coombs RW, Jones M, et al. Risk factors for repeatedly reactive HIV-1 EIA and indeterminate western blots. A population-based case-control study. Arch Intern Med, 1994, 154(5): 1129-1137.
17.	Riddler S, Mellors JW. Clinical use of Viral Load Measures. MMWR, April,1998.
18.	肖建春, 鄭啟唐, 趙之偉. HIV p24抗原檢測技術的研究進展. 國外醫學病毒學分冊, 2003, 10(4): 111-114.
19.	魏民. HIV實驗室檢測研究進展和發展趨勢. 國外醫學病毒學分冊, 2003, 10(3): 65-69.
20.	Mellors JW, Rinaldo CR, Gupta P, et al. Prognosis in HIV-1 Infection Predicted by the Quantity of virus in plasma. Science, 1996, 272(5): 1167.
21.	Lindback S, Karlsson AC, Mittler J, et al. Viral dynamics in primary HIV-1 infection. AIDS, 2000, 14(15): 2283-2291.
22.	Mellors JW, Munoz A, Giorgi JV, et al. Plasma Viral Load and CD4+ Lymphocytes as Prognostic Markers of HIV-1 Infection. Ann Intern Med, 1997, 126: 946-954.
23.	Abravaya K, Esping C, Hoenle R, et al. Performance of a Multiplex Qualitative PCR LCx Assay for Detection of Human Immunodeficiency Virus Type 1 (HIV-1) Group M Subtypes, Group O, and HIV-2. J Clin Microbiol, 2000, 38(2): 716-723.
24.	Zhao YQ, Min Y, Miller JW, et al. Quantification of Human Immundeficiency virus type 1 Proviral DNA by Using TaqMan Technology. J Chin Microbiol, 2002, 40(2): 675-678.
25.	Leroy V, Montcho C, Manigart O, et al. Maternal plasma viral load, zidovudine and mother-to-child transmission of HIV-1 in Africa:DITRAME ANRS 049a trial. AIDS, 2001, 15(4): 517-522.
26.	Deeks S, Beatty G, Cohen PT, et al. Viral load and CD4+T cells changes in patients failing potent protease inhibitor therapy. Presented at the 5th Conference on Retroviruses and Opportunistic Infections; February 1-5, 1998; Chicago. Abstract 419.
27.	肖瑤, 張可, 裴麗健, 等. 中國HIV/ AIDS 患者CD4+ CD8+T淋巴細胞與外周血各組份間關系的研究. 中國艾滋病性病, 2004, 10(2): 83-88.
28.	Edelstein RE, Nickerson DA, Tobe VO, et al. Oligonucleotide Ligation Assay for Detecting Mutations in the Human Immunodeficiency Virus Type 1 pol Gene That Are Associated with Resistance to Zidovudine, Didanosine, and Lamivudine. J Clin Microbiol, 1998, 36(7): 569-572.

1. Mylonakis E, Paliou M, Lally M, et al. Laboratory testing for infection with the human immunodeficiency virus:established and novel approaches. Am J Med, 2000, 109(7): 568-576.
2. Gurtler L, Muhlbacher A, Michl U, et al. Reduction of the diagnostic window with a new combined p24 antigen and human immunodeficiency virus antibody screening assay. J Virol Methods, 1998, 75(1): 27-38.
3. Weber B, Berger A, Rabenau H, et al. Evaluation of a New Combined Antigen and Antibody Human Immunodeficiency Virus Screening Assay ,VIDAS HIV DUO Ultra. J Clin Microbiol, 2002, 40(4): 1420-1426.
4. Weber B, Gürtler L, Thorstensson R, et al. Multicenter Evaluation of a New Automated Fourth-generation Human Immunodeficiency Virus Screening Assay with a sensitive Antigen Detection Module and High Specificity. J Clin Microbiol, 2002, 40(6): 1938-1946.
5. Gurtler LG, Eberle J, Lorbeer B, et al. Sensitivity and specificity of commercial ELISA kits for screening anti-LAV/HTLV III. J Virol Methods, 1987, 15(1): 11-23.
6. Petricciani JC, Epstein JS. The effects of the AIDS epidemic on the safety of the nation’s blood supply. Public Health Rep, 1988, 103(3): 236-241.
7. Barbe F, Klein M, Badonnel Y. Early detection of antibodies to human immunodeficiency virus 1 by a third-generation enzyme immunoassay.A comparative study with the results of second-generation immunoassays and western blot. Ann Biol Clin(Paris), 1994, 52(5): 341-345.
8. Suligoi B, Galli C, Massi M, et al. Precision and Accuracy of a Procedure for Detecting Recent Human Immunodeficiency Virus Infections by Calculating the Antibody Avidity Index by an Automated Immunoassay-Based Method. J Clin. Microbiol, 2002, 40(11): 4015-4020.
9. Ly TD, Laperche S, Brennan C, et al. Evaluation of the sensitivity and specificity of six HIV combined p24 antigen and antibody assays. J Virol Methods, 2004, 122(2): 185-194.
10. Bernard M Branson, MD. Rapid Test for HIV Antiboby. AIDS Reviews, 2000, 2: 76-83.
11. 魏經新. 免疫層析試驗檢測抗HIV1+2的臨床應用價值. 中國艾滋病性病, 2003, 9(5): 280-281.
12. 鄭曉麗. HIV檢測研究進展與發展趨勢. 現代檢驗醫學雜志, 2004, 19(6): 64-67.
13. 馮曉英, 徐曉琴. 吸毒人群尿液血液標本HIV-1抗體ELISA檢測對比分析. 中國性病艾滋病, 2003, 9(3): 132-133.
14. 趙立慶, 雷素萍, 李連學, 等. ELISA法檢測尿液中HIV-1抗體的研究分析. 中國艾滋病性病, 2003, 9(5): 276-277.
15. Nguyen KA, Busch MP. Evolving strategies for diagnosing human immunodeficiency virus infection. Am J Med, 2000, 109(7): 595-597.
16. Celum CL, Coombs RW, Jones M, et al. Risk factors for repeatedly reactive HIV-1 EIA and indeterminate western blots. A population-based case-control study. Arch Intern Med, 1994, 154(5): 1129-1137.
17. Riddler S, Mellors JW. Clinical use of Viral Load Measures. MMWR, April,1998.
18. 肖建春, 鄭啟唐, 趙之偉. HIV p24抗原檢測技術的研究進展. 國外醫學病毒學分冊, 2003, 10(4): 111-114.
19. 魏民. HIV實驗室檢測研究進展和發展趨勢. 國外醫學病毒學分冊, 2003, 10(3): 65-69.
20. Mellors JW, Rinaldo CR, Gupta P, et al. Prognosis in HIV-1 Infection Predicted by the Quantity of virus in plasma. Science, 1996, 272(5): 1167.
21. Lindback S, Karlsson AC, Mittler J, et al. Viral dynamics in primary HIV-1 infection. AIDS, 2000, 14(15): 2283-2291.
22. Mellors JW, Munoz A, Giorgi JV, et al. Plasma Viral Load and CD4+ Lymphocytes as Prognostic Markers of HIV-1 Infection. Ann Intern Med, 1997, 126: 946-954.
23. Abravaya K, Esping C, Hoenle R, et al. Performance of a Multiplex Qualitative PCR LCx Assay for Detection of Human Immunodeficiency Virus Type 1 (HIV-1) Group M Subtypes, Group O, and HIV-2. J Clin Microbiol, 2000, 38(2): 716-723.
24. Zhao YQ, Min Y, Miller JW, et al. Quantification of Human Immundeficiency virus type 1 Proviral DNA by Using TaqMan Technology. J Chin Microbiol, 2002, 40(2): 675-678.
25. Leroy V, Montcho C, Manigart O, et al. Maternal plasma viral load, zidovudine and mother-to-child transmission of HIV-1 in Africa:DITRAME ANRS 049a trial. AIDS, 2001, 15(4): 517-522.
26. Deeks S, Beatty G, Cohen PT, et al. Viral load and CD4+T cells changes in patients failing potent protease inhibitor therapy. Presented at the 5th Conference on Retroviruses and Opportunistic Infections; February 1-5, 1998; Chicago. Abstract 419.
27. 肖瑤, 張可, 裴麗健, 等. 中國HIV/ AIDS 患者CD4+ CD8+T淋巴細胞與外周血各組份間關系的研究. 中國艾滋病性病, 2004, 10(2): 83-88.
28. Edelstein RE, Nickerson DA, Tobe VO, et al. Oligonucleotide Ligation Assay for Detecting Mutations in the Human Immunodeficiency Virus Type 1 pol Gene That Are Associated with Resistance to Zidovudine, Didanosine, and Lamivudine. J Clin Microbiol, 1998, 36(7): 569-572.

《中國循證醫學雜志》

HIV/AIDS實驗室檢測及其研究進展

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《中國循證醫學雜志》

HIV/AIDS實驗室檢測及其研究進展

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