B淋巴細胞微粒數目在慢性阻塞性肺疾病患者支氣管肺泡灌洗液中的變化_《中國呼吸與危重監護雜志》

作者：

第三軍醫大學附屬西南醫院呼吸科(重慶 400038);

關鍵詞：

慢性阻塞性肺疾病 B淋巴細胞微粒支氣管肺泡灌洗液急性加重

DOI：

10.7507/1671-6205.2014032

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目的初步探討B淋巴細胞微粒(BLMPs)數目在慢性阻塞性肺疾病(簡稱慢阻肺)患者支氣管肺泡灌洗液(BALF)中的變化情況,并分析其數目變化與疾病分期可能存在的關系。方法選取2012年3月至2013年3月在西南醫院、新橋醫院、重慶醫科大學第一附屬醫院呼吸科就診的慢阻肺急性加重期患者33例,慢阻肺穩定期患者12例以及西南醫院健康體檢中心或者纖維支氣管鏡中心的對照者31例。分別采集BALF,并將所采集的樣本進行離心處理后,用CD20抗人抗體進行標記,使用流式細胞技術檢測每100 μL灌洗液中BLMPs的數目。結果 BLMPs數目在三組之間有顯著差異(P＜0.05),其中慢阻肺急性加重組BLMPs數目低于對照組和慢阻肺穩定期組(P＜0.05);而慢阻肺穩定期組與對照組比較差異無統計學意義(P＞0.05)。BLMPs的數目與吸煙、性別、年齡、體表面積無關(P＞0.05)。結論 BLMPs的數目在慢阻肺患者BALF中降低,尤其在急性加重期降低更明顯,BLMPs數目的減少可能與疾病分期有關,吸煙、性別、年齡、體表面積對其數目的變化無影響。

引用本文： 但小蘋, 邱倩, 楊再興, 焦燕, 熊瑋. B淋巴細胞微粒數目在慢性阻塞性肺疾病患者支氣管肺泡灌洗液中的變化. 中國呼吸與危重監護雜志, 2013, 12(2): 125-129. doi: 10.7507/1671-6205.2014032 復制

1.	柳濤,蔡柏薔.慢性阻塞性肺疾病診斷、處理和預防全球策略(2011 年修訂版) 介紹.中國呼吸與危重監護雜志,2012,11:1-12.
2.	Shaykhiev R,Crystal RG.Innate Immunity and Chronic Obstructive Pulmonary Disease.Gerontology,2013,59:481-489.
3.	Gao J,Prasad N.Chronic obstructive pulmonary disease in China: the potential role of indacaterol.J Thorac Dis,2013,5:549-558.
4.	陸再英,鐘南山,主編.內科學.7版,北京:人民衛生出版社,2010:62.
5.	Yang C,Mwaikambo BR,Zhu T,et al.Lymphocytic microparticles inhibit angiogenesis by stimulating oxidative stress and negatively regulating VEGF-induced pathways.Am J Physiol Regul Integr Comp Physiol,2008,294:R467-476.
6.	Gyorgy B,Szabo TG,Pasztoi M,et al.Membranevesicles,currentstate-of-the-art: emergingrole of extracellularvesicles.Cell Mol Life Sci, 2011,68:2667-2688.
7.	Jaiswal R,Luk F,Gong J,et al.Microparticleconferredmicro RNA profiles--implications in the transfer and dominance of cancertraits.Mol Cancer,2012,11:37.
8.	焦燕,邱倩,楊再興,等.淋巴細胞微粒刺激AKT/Foxo1阻滯氣道上皮細胞周期的研究.第三軍醫大學學報,2012,24:2498-2502.
9.	QIU Q, Xiong W,Yang C,et al.Lymphocyte-derived Microparticles induce bronchial epithelial cells'pro-inflammatory cytokine production and apoptosis.Mol Immunol,2013,55:220-230.
10.	Rovina N,Koutsoukou A,Koulouris NG.Inflammation and Immune Response in COPD: Where Do We Stand? Mediators Inflamm,2013,2013:413735.
11.	Brusselle GG,Joos GF,Bracke KR.New insights into the immunology of chronic obstructive pulmonary disease.Lancet,2011,378:1015-1026.
12.	Guervilly C,Lacroix R,Forel JM,et al.Highlevels of circulatingleukocytemicroparticles are associated with betteroutcome in acuterespiratorydistresssyndrome.Crit Care,2011,15:R31.
13.	Takahashi T,Kobayashi S,Fujino N,et al.Increased circulating endothelial microparticles in COPD patients: a potential biomarker for COPD exacerbation susceptibility.Thorax,2012,67:1067-1074.
14.	Smyth LJ,Starkey C,Vestbo J,et al.CD4-Regulatory Cells in COPD Patients.Chest,2007,132:156-163.
15.	Morel O,Toti F,Hugel B,et al.Procoagulant microparticles: disrupting the vascular homeostasis equation? Arterioscler.Arterioscler Thromb Vasc Biol,2006,26:2594-2604.
16.	焦憲法, 王國斌,秦建領,等.支氣管肺泡灌洗液中端粒酶檢測臨床價值研究.中國肺癌雜志,2002,5:275-277.
17.	Jiang YY,Xiao W,Zhu MX,et al.The effect of human antibacterial peptide LL-37 in the pathogenesis of chronic obstructive pulmonary disease.Respir Med,2012,106:1680-1689.
18.	He H,Ding L,Sun B,et al.Role of galactomannan determinations in bronchoalveolar lavage fluid samples from critically ill patients with chronic obstructive pulmonary disease for the diagnosis of invasive pulmonary aspergillosis: a prospective study.Crit Care,2012,16:R138.
19.	Delabranche X,Berger A,Boisrame-Helms J,et al.Microparticles and infectious diseases.Med Mal Infect,2012,42:335-343.
20.	Jy W,Horstman LL,Ahn YS.Microparticlesize and its relation to composition,functionalactivity,and clinicalsignificance.Semin Thromb Hemost,2010,36:876-880.
21.	Diehl P,Aleker M,Helbing T,et al.Increased platelet,leukocyte and endothelial microparticles predict enhanced coagulation and vascular inflammation in pulmonary hypertension.J Thromb Thrombolysis,2011,31:173-179.
22.	Xue J,Kass DJ,Bon J,et al.Plasma B lymphocyte stimulator and B cell differentiationin idiopathic pulmonary fibrosis patients.J Immunol,2013,191:2089-2095.
23.	Vander Strate BW,Postma DS,Brandsma CA,et al.Cigarette smoke-induced emphysema: A role for the B cell?Am J Respir Crit Care Med,2006,173:751-758.
24.	Litsiou E,Semitekolou M,Galani IE,et al.CXCL13 production in B cells via Toll-like receptor/lymphotoxin receptor signaling is involved in lymphoid neogenesis in chronic obstructive pulmonary disease.Am J Respir Crit Care Med,2013,187:1194-1202.
25.	Baka Z,Senolt L,Vencovsky J,et al.Increased serum concentration of immune cell derived microparticles in polymyositis/dermatomyositis.Immunol Lett,2010,128:124-130.
26.	Miguet L,Bechade G,Fornecker L,et al.Proteomic analysis of malignant B-cellderivedmicroparticles reveals CD148 as a potentially useful antigenic biomarker for mantle cell lymphoma diagnosis.J Proteome Res,2009,8:3346-3354.
27.	Guiducci S,Ricci L,Romano E,et al.Microparticles and Kawasaki disease: a marker of vascular damage? Clin Exp Rheumatol,2011,29:S121-S125.
28.	王娜,張湘燕,張程,等.慢性阻塞性肺疾病患者免疫功能的變化.貴州醫藥,2011,35:593-595.
29.	McGorum BC,Dixon PM,Halliwell RE.Phenotypic analysis of peripheral blood and bronchoalveolar lavage fluid lymphocytes in control and chronic obstructivepulmonary disease affected horses,before and after natural (hay and straw) challenges.Vet Immunol Immunopathol,1993,36:207-222.
30.	李冠華,李廣生,趙芳,等.慢性阻塞性肺疾病急性加重期支氣管肺泡灌洗液的細胞分類特點分析.國際呼吸雜志,2012,16:32.
31.	Jy W, .Hor stamn LL, Ahn YS.Microparticle size and its relationto composition,functional activity,and clinical significance.Semin Thromb Hemost, 2010,36:876-880.
32.	Tantawy AA,Adly AA,Ismail EA,et al.Flow cytometric assessment of circulating platelet anderythrocytes microparticles in young thalassemia majorpatients: relation to pulmonary hypertension and aorticwall stiffness.Eur J Haematol, 2013,90:508-518.

1. 柳濤,蔡柏薔.慢性阻塞性肺疾病診斷、處理和預防全球策略(2011 年修訂版) 介紹.中國呼吸與危重監護雜志,2012,11:1-12.
2. Shaykhiev R,Crystal RG.Innate Immunity and Chronic Obstructive Pulmonary Disease.Gerontology,2013,59:481-489.
3. Gao J,Prasad N.Chronic obstructive pulmonary disease in China: the potential role of indacaterol.J Thorac Dis,2013,5:549-558.
4. 陸再英,鐘南山,主編.內科學.7版,北京:人民衛生出版社,2010:62.
5. Yang C,Mwaikambo BR,Zhu T,et al.Lymphocytic microparticles inhibit angiogenesis by stimulating oxidative stress and negatively regulating VEGF-induced pathways.Am J Physiol Regul Integr Comp Physiol,2008,294:R467-476.
6. Gyorgy B,Szabo TG,Pasztoi M,et al.Membranevesicles,currentstate-of-the-art: emergingrole of extracellularvesicles.Cell Mol Life Sci, 2011,68:2667-2688.
7. Jaiswal R,Luk F,Gong J,et al.Microparticleconferredmicro RNA profiles--implications in the transfer and dominance of cancertraits.Mol Cancer,2012,11:37.
8. 焦燕,邱倩,楊再興,等.淋巴細胞微粒刺激AKT/Foxo1阻滯氣道上皮細胞周期的研究.第三軍醫大學學報,2012,24:2498-2502.
9. QIU Q, Xiong W,Yang C,et al.Lymphocyte-derived Microparticles induce bronchial epithelial cells'pro-inflammatory cytokine production and apoptosis.Mol Immunol,2013,55:220-230.
10. Rovina N,Koutsoukou A,Koulouris NG.Inflammation and Immune Response in COPD: Where Do We Stand? Mediators Inflamm,2013,2013:413735.
11. Brusselle GG,Joos GF,Bracke KR.New insights into the immunology of chronic obstructive pulmonary disease.Lancet,2011,378:1015-1026.
12. Guervilly C,Lacroix R,Forel JM,et al.Highlevels of circulatingleukocytemicroparticles are associated with betteroutcome in acuterespiratorydistresssyndrome.Crit Care,2011,15:R31.
13. Takahashi T,Kobayashi S,Fujino N,et al.Increased circulating endothelial microparticles in COPD patients: a potential biomarker for COPD exacerbation susceptibility.Thorax,2012,67:1067-1074.
14. Smyth LJ,Starkey C,Vestbo J,et al.CD4-Regulatory Cells in COPD Patients.Chest,2007,132:156-163.
15. Morel O,Toti F,Hugel B,et al.Procoagulant microparticles: disrupting the vascular homeostasis equation? Arterioscler.Arterioscler Thromb Vasc Biol,2006,26:2594-2604.
16. 焦憲法, 王國斌,秦建領,等.支氣管肺泡灌洗液中端粒酶檢測臨床價值研究.中國肺癌雜志,2002,5:275-277.
17. Jiang YY,Xiao W,Zhu MX,et al.The effect of human antibacterial peptide LL-37 in the pathogenesis of chronic obstructive pulmonary disease.Respir Med,2012,106:1680-1689.
18. He H,Ding L,Sun B,et al.Role of galactomannan determinations in bronchoalveolar lavage fluid samples from critically ill patients with chronic obstructive pulmonary disease for the diagnosis of invasive pulmonary aspergillosis: a prospective study.Crit Care,2012,16:R138.
19. Delabranche X,Berger A,Boisrame-Helms J,et al.Microparticles and infectious diseases.Med Mal Infect,2012,42:335-343.
20. Jy W,Horstman LL,Ahn YS.Microparticlesize and its relation to composition,functionalactivity,and clinicalsignificance.Semin Thromb Hemost,2010,36:876-880.
21. Diehl P,Aleker M,Helbing T,et al.Increased platelet,leukocyte and endothelial microparticles predict enhanced coagulation and vascular inflammation in pulmonary hypertension.J Thromb Thrombolysis,2011,31:173-179.
22. Xue J,Kass DJ,Bon J,et al.Plasma B lymphocyte stimulator and B cell differentiationin idiopathic pulmonary fibrosis patients.J Immunol,2013,191:2089-2095.
23. Vander Strate BW,Postma DS,Brandsma CA,et al.Cigarette smoke-induced emphysema: A role for the B cell?Am J Respir Crit Care Med,2006,173:751-758.
24. Litsiou E,Semitekolou M,Galani IE,et al.CXCL13 production in B cells via Toll-like receptor/lymphotoxin receptor signaling is involved in lymphoid neogenesis in chronic obstructive pulmonary disease.Am J Respir Crit Care Med,2013,187:1194-1202.
25. Baka Z,Senolt L,Vencovsky J,et al.Increased serum concentration of immune cell derived microparticles in polymyositis/dermatomyositis.Immunol Lett,2010,128:124-130.
26. Miguet L,Bechade G,Fornecker L,et al.Proteomic analysis of malignant B-cellderivedmicroparticles reveals CD148 as a potentially useful antigenic biomarker for mantle cell lymphoma diagnosis.J Proteome Res,2009,8:3346-3354.
27. Guiducci S,Ricci L,Romano E,et al.Microparticles and Kawasaki disease: a marker of vascular damage? Clin Exp Rheumatol,2011,29:S121-S125.
28. 王娜,張湘燕,張程,等.慢性阻塞性肺疾病患者免疫功能的變化.貴州醫藥,2011,35:593-595.
29. McGorum BC,Dixon PM,Halliwell RE.Phenotypic analysis of peripheral blood and bronchoalveolar lavage fluid lymphocytes in control and chronic obstructivepulmonary disease affected horses,before and after natural (hay and straw) challenges.Vet Immunol Immunopathol,1993,36:207-222.
30. 李冠華,李廣生,趙芳,等.慢性阻塞性肺疾病急性加重期支氣管肺泡灌洗液的細胞分類特點分析.國際呼吸雜志,2012,16:32.
31. Jy W, .Hor stamn LL, Ahn YS.Microparticle size and its relationto composition,functional activity,and clinical significance.Semin Thromb Hemost, 2010,36:876-880.
32. Tantawy AA,Adly AA,Ismail EA,et al.Flow cytometric assessment of circulating platelet anderythrocytes microparticles in young thalassemia majorpatients: relation to pulmonary hypertension and aorticwall stiffness.Eur J Haematol, 2013,90:508-518.

《中國呼吸與危重監護雜志》

B淋巴細胞微粒數目在慢性阻塞性肺疾病患者支氣管肺泡灌洗液中的變化

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《中國呼吸與危重監護雜志》

B淋巴細胞微粒數目在慢性阻塞性肺疾病患者支氣管肺泡灌洗液中的變化

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