血管基質片段輔助游離脂肪移植早期移植物組織學變化及相關實驗研究_《中國修復重建外科雜志》

作者：

袁藝 , 魯峰 , 高建華

南方醫科大學南方醫院整形外科（廣州，510515）;

關鍵詞：

血管基質片段游離脂肪移植血管生成生長因子裸鼠

DOI：

10.7507/1002-1892.20130103

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目的探討血管基質片段（vascular stromal fraction，SVF）輔助游離脂肪移植促進其早期存活的相關機制。方法取5例行腹部抽脂手術女性患者自愿捐贈的脂肪組織分離、培養SVF。4～6周齡裸鼠24只（體重15～18 g），將裸鼠背部左、右側隨機分為A、B組（n=24），采用Coleman技術分別于A、B組裸鼠背部皮下移植脂肪0.5 mL和含5 × 105個SVF的脂肪0.5 mL。移植后1、3、5、7 d分別取6只裸鼠，取出移植脂肪組織，行大體觀察、CD31免疫組織化學染色及Western blot檢測分析。結果隨著移植時間增加，A、B組移植脂肪組織濕重均呈上升趨勢，但各時間點A、B組間比較差異均無統計學意義（P gt; 0.05）。移植后1、3 d兩組均未見CD31陽性細胞；5、7 d B組CD31陽性細胞數顯著高于A組（P lt; 0.05），兩組7 d時CD31陽性細胞數顯著高于5 d時（P lt; 0.05）。Western blot檢測示，兩組移植脂肪中VEGF蛋白表達在移植后第3天達峰值，隨后開始下降；1、3、5 d B組VEGF蛋白表達明顯高于A組（P lt; 0.05）。兩組移植脂肪中HGF蛋白表達在移植后前5 d均保持在較高水平，7 d開始下降，B組均明顯高于A組（P lt; 0.05）。結論在游離脂肪移植早期，SVF通過旁分泌生長因子促進移植脂肪血管生成。

引用本文： 袁藝,魯峰,高建華. 血管基質片段輔助游離脂肪移植早期移植物組織學變化及相關實驗研究. 中國修復重建外科雜志, 2013, 27(4): 449-453. doi: 10.7507/1002-1892.20130103 復制

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2.	Yamaguchi T, Sawa Y, Miyamoto Y, et al. Therapeutic angiogenesis induced by injecting hepatocyte growth factor in ischemic canine hearts. Surg Today, 2005, 35(10): 855-860.
3.	Miyagawa S, Sawa Y, Fukuda K, et al. Angiogenic gene cell therapy using suicide gene system regulates the effect of angiogenesis in infarcted rat heart. Transplantation, 2006, 81(6): 902-907.
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5.	Yoshimura K, Shigeura T, Matsumoto D, et al. Characterization of freshly isolated and cultured cells derived from the fatty and fluid portions of liposuction aspirates. J Cell Physiol, 2006, 208(1): 64-76.
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8.	Zuk PA, Zhu M, Ashjian P, et al. Human adipose tissue is a source of multipotent stem cells. Mol Biol Cell, 2002, 13(12): 4279-4295.
9.	Tallone T, Realini C, Bohmler A, et al. Adult human adipose tissue contains several types of multipotent cells. J Cardiovasc Transl Res, 2011, 4(2): 200-210.
10.	Wilson A, Butler PE, Seifalian AM. Adipose-derived stem cells for clinical applications: a review. Cell Prolif, 2011, 44(1): 86-98.
11.	Shiffman MA, Mirrafati S. Fat transfer techniques: the effect of harvest and transfer methods on adipocyte viability and review of the literature. Dermatol Surg, 2001, 27(9): 819-826.
12.	Christiaens V, Lijnen HR. Angiogenesis and development of adipose tissue. Mol Cell Endocrinol, 2010, 318(1-2): 2-9.
13.	Yoshimura K, Suga H, Eto H. Adipose-derived stem/progenitor cells: roles in adipose tissue remodeling and potential use for soft tissue augmentation. Regen Med, 2009, 4(2): 265-273.
14.	Lee EY, Xia Y, Kim WS, et al. Hypoxia-enhanced wound-healing function of adipose-derived stem cells: increase in stem cell proliferation and up-regulation of VEGF and bFGF. Wound Repair Regen, 2009, 17(4): 540-547.
15.	Rubina K, Kalinina N, Efimenko A, et al. Adipose stromal cells stimulate angiogenesis via promoting progenitor cell differentiation, secretion of angiogenic factors, and enhancing vessel maturation. Tissue Eng Part A, 2009, 15(8): 2039-2050.
16.	Eto H, Kato H, Suga H, et al. The fate of adipocytes after nonvascularized fat grafting: evidence of early death and replacement of adipocytes. Plast Reconstr Surg, 2012, 129(5): 1081-1092.
17.	Maumus M, Peyrafitte JA, D’Angelo R, et al. Native human adipose stromal cells: localization, morphology and phenotype. Int J Obes (Lond), 2011, 35(9): 1141-1153.
18.	Traktuev DO, Prater DN, Merfeld-Clauss S, et al. Robust functional vascular network formation in vivo by cooperation of adipose progenitor and endothelial cells. Circ Res, 2009, 104(12): 1410-1420.
19.	Butala P, Hazen A, Szpalski C, et al. Endogenous stem cell therapy enhances fat graft survival. Plast Reconstr Surg, 2012, 130(2): 293-306.

1. Risau W. Mechanisms of angiogenesis. Nature, 1997, 386(6626): 671-674.
2. Yamaguchi T, Sawa Y, Miyamoto Y, et al. Therapeutic angiogenesis induced by injecting hepatocyte growth factor in ischemic canine hearts. Surg Today, 2005, 35(10): 855-860.
3. Miyagawa S, Sawa Y, Fukuda K, et al. Angiogenic gene cell therapy using suicide gene system regulates the effect of angiogenesis in infarcted rat heart. Transplantation, 2006, 81(6): 902-907.
4. Taniyama Y, Morishita R, Aoki M, et al. Therapeutic angiogenesis induced by human hepatocyte growth factor gene in rat and rabbit hindlimb ischemia models: preclinical study for treatment of peripheral arterial disease. Gene Ther, 2001, 8(3): 181-189.
5. Yoshimura K, Shigeura T, Matsumoto D, et al. Characterization of freshly isolated and cultured cells derived from the fatty and fluid portions of liposuction aspirates. J Cell Physiol, 2006, 208(1): 64-76.
6. Matsumoto D, Sato K, Gonda K, et al. Cell-assisted lipotransfer: Supportive use of human adipose-derived cells for soft tissue augmentation with lipoinjection. Tissue Eng, 2006, 12(12): 3375-3382.
7. Yoshimura K, Sato K, Aoi N, et al. Cell-assisted lipotransfer for facial lipoatrophy: efficacy of clinical use of adipose-derived stem cells. Dermatol Surg, 2008, 34(9): 1178-1185.
8. Zuk PA, Zhu M, Ashjian P, et al. Human adipose tissue is a source of multipotent stem cells. Mol Biol Cell, 2002, 13(12): 4279-4295.
9. Tallone T, Realini C, Bohmler A, et al. Adult human adipose tissue contains several types of multipotent cells. J Cardiovasc Transl Res, 2011, 4(2): 200-210.
10. Wilson A, Butler PE, Seifalian AM. Adipose-derived stem cells for clinical applications: a review. Cell Prolif, 2011, 44(1): 86-98.
11. Shiffman MA, Mirrafati S. Fat transfer techniques: the effect of harvest and transfer methods on adipocyte viability and review of the literature. Dermatol Surg, 2001, 27(9): 819-826.
12. Christiaens V, Lijnen HR. Angiogenesis and development of adipose tissue. Mol Cell Endocrinol, 2010, 318(1-2): 2-9.
13. Yoshimura K, Suga H, Eto H. Adipose-derived stem/progenitor cells: roles in adipose tissue remodeling and potential use for soft tissue augmentation. Regen Med, 2009, 4(2): 265-273.
14. Lee EY, Xia Y, Kim WS, et al. Hypoxia-enhanced wound-healing function of adipose-derived stem cells: increase in stem cell proliferation and up-regulation of VEGF and bFGF. Wound Repair Regen, 2009, 17(4): 540-547.
15. Rubina K, Kalinina N, Efimenko A, et al. Adipose stromal cells stimulate angiogenesis via promoting progenitor cell differentiation, secretion of angiogenic factors, and enhancing vessel maturation. Tissue Eng Part A, 2009, 15(8): 2039-2050.
16. Eto H, Kato H, Suga H, et al. The fate of adipocytes after nonvascularized fat grafting: evidence of early death and replacement of adipocytes. Plast Reconstr Surg, 2012, 129(5): 1081-1092.
17. Maumus M, Peyrafitte JA, D’Angelo R, et al. Native human adipose stromal cells: localization, morphology and phenotype. Int J Obes (Lond), 2011, 35(9): 1141-1153.
18. Traktuev DO, Prater DN, Merfeld-Clauss S, et al. Robust functional vascular network formation in vivo by cooperation of adipose progenitor and endothelial cells. Circ Res, 2009, 104(12): 1410-1420.
19. Butala P, Hazen A, Szpalski C, et al. Endogenous stem cell therapy enhances fat graft survival. Plast Reconstr Surg, 2012, 130(2): 293-306.

《中國修復重建外科雜志》

血管基質片段輔助游離脂肪移植早期移植物組織學變化及相關實驗研究

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《中國修復重建外科雜志》

血管基質片段輔助游離脂肪移植早期移植物組織學變化及相關實驗研究

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