The Study of Skp2 Expression in Human Testicular Germ Cell Tumors_West China Medical Journal

Authors：

FU Guangqing ¹ , HUANG Jin ² , LIANG Yong ¹ , HUANG Fengming ¹ , HAN Dengjun. ¹

1. Department of Urology, Zigong No. 4 People’s Hospital, Zigong, Sichuan 643000, P. R. China; 2. West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China;

Corresponding?author：

Keywords：

S期激酶相關蛋白2; 泛素-蛋白酶體途徑; 睪丸生殖細胞腫瘤; 免疫組織化學

DOI：

10.7507/1002-0179.20130318

Video：

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目的　通過檢測人睪丸生殖細胞腫瘤中的Skp2蛋白質異常表達,探討相關意義。方法　應用S-P免疫組織化學法檢測睪丸生殖細胞腫瘤，正常睪丸組織和慢性睪丸炎組織中Skp2的表達。結果　睪丸生殖細胞腫瘤中Skp2陽性表達率為74.5%，正常睪丸組織中Skp2陽性表達率為20.0%，在慢性睪丸炎組織中Skp2陽性表達率為40.0%，在3種不同睪丸組織中表達差異有統計學意義（P＜0.05）；Skp2表達與不同組織學類型的睪丸生殖細胞腫瘤無相關性（P＞0.05）；隨著臨床分期的增高，睪丸生殖細胞腫瘤中的Skp2表達增多，差異無統計學意義（P＞0.05）。結論　在人睪丸生殖細胞腫瘤中的Skp2高表達，提示細胞周期的異常調控在睪丸生殖細胞腫瘤的發生、分化中起著重要的作用。

Citation： FU Guangqing,HUANG Jin,LIANG Yong,HUANG Fengming,HAN Dengjun.. The Study of Skp2 Expression in Human Testicular Germ Cell Tumors. West China Medical Journal, 2013, 28(7): 1029-1032. doi: 10.7507/1002-0179.20130318 Copy

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2.	Remmele W, Stegner HE. Recommendation for uniform definition of an immunoreactive score (IRS) for immunohistochemical estrogen receptor detection (ER-ICA) in breast cancer tissue[J]. Pathologe, 1987, 8(3): 138-140.
3.	何洪智, 趙曉航, 張立勇, 等. 蛋白質泛素化降解途徑與腫瘤發生的關系[J]. 世界華人消化雜志, 2002, 10(12): 1365-1368.
4.	羅四維, 彭先兵, 張群, 等. Skp2結構與腫瘤關系研究進展[J]. 中華實用診斷與治療雜志, 2010, 24(2): 110-112.
5.	Latres E, Chiarle R, Schulman BA, et al. Role of the F-box protein Skp2 in lymphomagenesis[J]. Proc Natl Acad Sci USA, 2001, 98(5): 2515-2520.
6.	趙君, 楊春鹿, 趙蘇英. 肺癌中細胞S期激酶相關蛋白2的表達及其對預后的影響[J]. 中華腫瘤雜志, 2007, 29(4): 289-292.
7.	Masuda TA, Inoue H, Sonoda H, et al. Clinical and biological significance of S-phase kinase-associated protein 2 (Skp2) gene expression in gastric carcinoma modulation of malignant phenotype by Skp20 over expression possibly via p27 proteolysis[J]. Cancer Res, 2002, 62(13): 3819-3825.
8.	Yang G, Ayala G, De Marzo A, et al. Elevated Skp2 protein expression in human prostate Cancer: association with loss of the cyclin-dependent kinase inhibitor p27 and PTEN and with reduced recurrence-free survival[J]. Clin Cancer Res, 2002, 8(11): 3419-3426.
9.	蔣國良. Skp2蛋白在卵巢上皮性腫瘤中的表達及意義[J]. 蘇州醫學, 2010, 33(2): 87-90.
10.	隋戰勝, 李香波, 李香玉, 等. 應用組織芯片技術研究Skp2、p27在膀胱移行細胞癌中的表達及意義[J]. 臨床外科雜志, 2011, 19(7): 483-485.
11.	Chiappetta G, De Marco C, Quintiero A, et al. Overexpression of the S-phase kinase-associated protein 2 in thyroid cancer[J]. Endocr Relat Cancer, 2007, 14(2): 405-420.
12.	Koga H, Harada M, Ohtsubo M, et al. Troglitazone induces p27Kip1-associated cell-cycle arrest through down-regulating Skp2 in human hepatoma cells[J]. Hepatology, 2003, 37(5): 1086-1096.
13.	Lin R, Wang TT, Miller WH Jr, et al. Inhibition of F-Box protein p45(SKP2) expression and stabilization of cyclin-dependent kinase inhibitor p27(KIP1) in vitamin D analog-treated cancer cells[J]. Endocrinology, 2003, 144(3): 749-753.
14.	Ishii T, Matsuse T, Masuda M, et al. The effects of S-phase kinase-associated protein 2 (SKP2) on cell cycle status, viability, and chemoresistance in A549 lung adenocarcinoma cells[J]. Exp Lung Res, 2004, 30(8): 687-703.
15.	呂會增, 朱曉峰. Skp2,p27蛋白表達與胃癌的臨床關系[J]. 中國醫師雜志, 2005, 7(6): 751-753.
16.	Dowen SE, Scott A, Mukherjee G, et al. Overexpression of Skp2 in carcinoma of the cervix does not correlate inversely with p27 expression[J]. Int J Cancer, 2003, 105(3): 326-330.
17.	Sui L, Dong Y, Watanabe Y, et al. Clinical significance of Skp2 expression,alone an d combined with Jabl and p27 inepithelial ovarian tumors[J]. Oncol Rep, 2006, 15(4): 765-771.

1. 　吳階平. 吳階平泌尿外科學[M]. 濟南: 山東科學技術出版社, 2003: 993.
2. 　Remmele W, Stegner HE. Recommendation for uniform definition of an immunoreactive score (IRS) for immunohistochemical estrogen receptor detection (ER-ICA) in breast cancer tissue[J]. Pathologe, 1987, 8(3): 138-140.
3. 　何洪智, 趙曉航, 張立勇, 等. 蛋白質泛素化降解途徑與腫瘤發生的關系[J]. 世界華人消化雜志, 2002, 10(12): 1365-1368.
4. 　羅四維, 彭先兵, 張群, 等. Skp2結構與腫瘤關系研究進展[J]. 中華實用診斷與治療雜志, 2010, 24(2): 110-112.
5. 　Latres E, Chiarle R, Schulman BA, et al. Role of the F-box protein Skp2 in lymphomagenesis[J]. Proc Natl Acad Sci USA, 2001, 98(5): 2515-2520.
6. 　趙君, 楊春鹿, 趙蘇英. 肺癌中細胞S期激酶相關蛋白2的表達及其對預后的影響[J]. 中華腫瘤雜志, 2007, 29(4): 289-292.
7. 　Masuda TA, Inoue H, Sonoda H, et al. Clinical and biological significance of S-phase kinase-associated protein 2 (Skp2) gene expression in gastric carcinoma modulation of malignant phenotype by Skp20 over expression possibly via p27 proteolysis[J]. Cancer Res, 2002, 62(13): 3819-3825.
8. 　Yang G, Ayala G, De Marzo A, et al. Elevated Skp2 protein expression in human prostate Cancer: association with loss of the cyclin-dependent kinase inhibitor p27 and PTEN and with reduced recurrence-free survival[J]. Clin Cancer Res, 2002, 8(11): 3419-3426.
9. 　蔣國良. Skp2蛋白在卵巢上皮性腫瘤中的表達及意義[J]. 蘇州醫學, 2010, 33(2): 87-90.
10. 　隋戰勝, 李香波, 李香玉, 等. 應用組織芯片技術研究Skp2、p27在膀胱移行細胞癌中的表達及意義[J]. 臨床外科雜志, 2011, 19(7): 483-485.
11. 　Chiappetta G, De Marco C, Quintiero A, et al. Overexpression of the S-phase kinase-associated protein 2 in thyroid cancer[J]. Endocr Relat Cancer, 2007, 14(2): 405-420.
12. 　Koga H, Harada M, Ohtsubo M, et al. Troglitazone induces p27Kip1-associated cell-cycle arrest through down-regulating Skp2 in human hepatoma cells[J]. Hepatology, 2003, 37(5): 1086-1096.
13. 　Lin R, Wang TT, Miller WH Jr, et al. Inhibition of F-Box protein p45(SKP2) expression and stabilization of cyclin-dependent kinase inhibitor p27(KIP1) in vitamin D analog-treated cancer cells[J]. Endocrinology, 2003, 144(3): 749-753.
14. 　Ishii T, Matsuse T, Masuda M, et al. The effects of S-phase kinase-associated protein 2 (SKP2) on cell cycle status, viability, and chemoresistance in A549 lung adenocarcinoma cells[J]. Exp Lung Res, 2004, 30(8): 687-703.
15. 　呂會增, 朱曉峰. Skp2,p27蛋白表達與胃癌的臨床關系[J]. 中國醫師雜志, 2005, 7(6): 751-753.
16. 　Dowen SE, Scott A, Mukherjee G, et al. Overexpression of Skp2 in carcinoma of the cervix does not correlate inversely with p27 expression[J]. Int J Cancer, 2003, 105(3): 326-330.
17. 　Sui L, Dong Y, Watanabe Y, et al. Clinical significance of Skp2 expression,alone an d combined with Jabl and p27 inepithelial ovarian tumors[J]. Oncol Rep, 2006, 15(4): 765-771.

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