CD14對胃癌細胞核轉錄因子-κB活性及人β防御素-2表達的影響_《華西醫學》

作者：

 李康 , 旦增 , 旺加 , 剛組 , 澤永革

西藏自治區人民醫院消化內科（拉薩，850000）;

關鍵詞：

CD14 胃癌細胞核轉錄因子-κB 人β防御素-2

DOI：

10.7507/1002-0179.20130107

視頻：

導出 下載 收藏 掃碼 引用

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

目的　研究CD14過表達對胃癌細胞核轉錄因子-κB（NF-κB）活性以及人β防御素-2（hBD-2）表達的影響，探討CD14在胃癌發生發展中的作用。方法　體外培養CD14穩定轉染的胃癌SGC-7901細胞系及空質粒轉染的對照細胞，CD14蛋白受體胞壁酰二肽刺激細胞，凝膠遷移實驗檢測NF-κB的活性，蛋白質印跡法檢測NF-κB蛋白的表達，同時分別用逆轉錄-聚合酶鏈反應以及蛋白質印跡法檢測hBD-2 mRNA及蛋白的表達。結果　與對照組相比，CD14過表達的細胞中NF-κB的活性明顯增強，蛋白表達量也大幅度增加，同時hBD-2的mRNA及蛋白的表達都有所提高。結論　胃癌細胞中CD14在介導NF-κB的激活以及hBD-2的表達中發揮重要作用。

引用本文： 李康,旦增,旺加,剛組,澤永革. CD14對胃癌細胞核轉錄因子-κB活性及人β防御素-2表達的影響. 華西醫學, 2013, 28(3): 323-326. doi: 10.7507/1002-0179.20130107 復制

1.	Ferlay J, Shin HR, Bray F, et al. Estimates of worldwide burden of cancer in 2008: GLOBOCAN 2008[J]. Int J Cancer, 2010, 127(12): 2893-2917.
2.	Zhao D, Sun T, Zhang X, et al. Role of CD14 Promoter Polymorphisms in Helicobacter pylori Infection Related Gastric Carcinoma[J]. Clin Cancer Res, 2007, 13(8): 2362-2368.
3.	Goyert SM, Ferrero E, Rettig WJ, et al. The CD14 monocyte differentiation antigen maps to a region encoding growth factors and receptors[J]. Science, 1988, 239(4839): 497-500.
4.	Zhang DE, Hetherington CJ, Tan S, et al. Sp1 is a critical factor for the monocytic specific expression of human CD14[J]. J Biol Chem,1994, 269(15): 11425-11434.
5.	Netea MG, Kullberg BJ, van der Meer JW. Lipopolysaccharide induced production of tumour necrosis factor and interleukin-1 is differentially regulated at the receptor level:the role of CD14-dependent and CD14-independent pathways[J]. Immunology,1998, 94(3): 340-344.
6.	Hold GL, Rabkin CS, Gammon MD, et al. CD14-159C/T and TLR9-1237T/C polymorphisms are not associated with gastric cancer risk in Caucasian populations[J]. Eur J Cancer Prev, 2009, 18(2): 117-119.
7.	Miyake K. Innate immune sensing of pathogens and danger signals by cell surface Toll-like receptors[J]. Semin Immunol, 2007, 19(1): 3-10.
8.	Li K, Dan Z, Hu X, et al. CD14 overexpression upregulates TNF-a-mediated inflammatory responses and suppresses the malignancy of gastric carcinoma cells[J]. Mol Cell Biochem, 2013, 376(1-2): 137-143.
9.	Midorikawa K, Ouhara K, Komatsuzawa H, et al. Staphylococcus aureus susceptibility to innate antimicrobial peptides, beta-defensins and CAP18, expressed by human keratinocytes[J]. Infect Immum, 2003, 71(7): 3730-3739.
10.	Hiratsuka T, Mukae H, Iiboshi H, et al. Increased concentrations of human beta-defensins in plasma and bronchoalveolar lavage fluid of patients with diffuse panbrochiolitis[J]. Thorax, 2003, 58(5): 425-430.
11.	Tsutsumi-Ishii Y, Nagaoka I. NF-kappa B mediated transcriptional regulation of human beta- defensin-2 gene following lipopolysaccharide stimulation[J]. J Leukoc Biol, 2002, 71(1): 154-162.
12.	Tomita T, Nagase T, Ohga E, et al. Molecular mechanisms underlying human beta-defensin-2 gene expression in a human airway cell line(LC2/ad)[J]. Respirology, 2002, 7(4): 305-310.

1. 　Ferlay J, Shin HR, Bray F, et al. Estimates of worldwide burden of cancer in 2008: GLOBOCAN 2008[J]. Int J Cancer, 2010, 127(12): 2893-2917.
2. 　Zhao D, Sun T, Zhang X, et al. Role of CD14 Promoter Polymorphisms in Helicobacter pylori Infection Related Gastric Carcinoma[J]. Clin Cancer Res, 2007, 13(8): 2362-2368.
3. 　Goyert SM, Ferrero E, Rettig WJ, et al. The CD14 monocyte differentiation antigen maps to a region encoding growth factors and receptors[J]. Science, 1988, 239(4839): 497-500.
4. 　Zhang DE, Hetherington CJ, Tan S, et al. Sp1 is a critical factor for the monocytic specific expression of human CD14[J]. J Biol Chem,1994, 269(15): 11425-11434.
5. 　Netea MG, Kullberg BJ, van der Meer JW. Lipopolysaccharide induced production of tumour necrosis factor and interleukin-1 is differentially regulated at the receptor level:the role of CD14-dependent and CD14-independent pathways[J]. Immunology,1998, 94(3): 340-344.
6. 　Hold GL, Rabkin CS, Gammon MD, et al. CD14-159C/T and TLR9-1237T/C polymorphisms are not associated with gastric cancer risk in Caucasian populations[J]. Eur J Cancer Prev, 2009, 18(2): 117-119.
7. 　Miyake K. Innate immune sensing of pathogens and danger signals by cell surface Toll-like receptors[J]. Semin Immunol, 2007, 19(1): 3-10.
8. 　Li K, Dan Z, Hu X, et al. CD14 overexpression upregulates TNF-a-mediated inflammatory responses and suppresses the malignancy of gastric carcinoma cells[J]. Mol Cell Biochem, 2013, 376(1-2): 137-143.
9. 　Midorikawa K, Ouhara K, Komatsuzawa H, et al. Staphylococcus aureus susceptibility to innate antimicrobial peptides, beta-defensins and CAP18, expressed by human keratinocytes[J]. Infect Immum, 2003, 71(7): 3730-3739.
10. 　Hiratsuka T, Mukae H, Iiboshi H, et al. Increased concentrations of human beta-defensins in plasma and bronchoalveolar lavage fluid of patients with diffuse panbrochiolitis[J]. Thorax, 2003, 58(5): 425-430.
11. 　Tsutsumi-Ishii Y, Nagaoka I. NF-kappa B mediated transcriptional regulation of human beta- defensin-2 gene following lipopolysaccharide stimulation[J]. J Leukoc Biol, 2002, 71(1): 154-162.
12. 　Tomita T, Nagase T, Ohga E, et al. Molecular mechanisms underlying human beta-defensin-2 gene expression in a human airway cell line(LC2/ad)[J]. Respirology, 2002, 7(4): 305-310.

下一篇
內鏡反轉黏膜切除術治療超低位直腸大型側向發育型腫瘤

《華西醫學》

CD14對胃癌細胞核轉錄因子-κB活性及人β防御素-2表達的影響

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

下一篇

Format

Content

《華西醫學》

CD14對胃癌細胞核轉錄因子-κB活性及人β防御素-2表達的影響

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

下一篇

Format

Content

摘要全文圖表視頻參考文獻施引文獻補充材料